Liquid chromatographic methods for the determination of direct oral anticoagulant drugs in biological samples: A critical review.

Liquid chromatographic methods for the determination of direct oral anticoagulant drugs in biological samples: A critical review. Anal Chim Acta. 2019 Oct 17;1076:18-31 Authors: Gouveia F, Bicker J, Gonçalves J, Alves G, Falcão A, Fortuna A Abstract Direct oral anticoagulants (DOACs) are first-line drugs used for the treatment of venous thromboembolism and prevention of stroke in patients with atrial fibrillation. These drugs do not require the regular biochemical monitoring that is mandatory for warfarin, and they exhibit shorter half-lives and have a faster onset of action. Since recent real-world studies evidence a higher prevalence of adverse side effects than what was anticipated in clinical trials, monitoring the plasma concentrations of DOACs is being used to personalize their pharmacotherapy, in accordance to the characteristics of the patient, and to evaluate the adherence to therapy. In order to fulfill the aforementioned clinical unmet needs, there are specific coagulation assays that indirectly assess the plasma concentrations of DOACs. Nevertheless, these assays are not sufficiently accurate or sensitive. For this reason, liquid chromatography techniques coupled to mass spectrometry detection, are considered the gold standard method to accurately determine plasma concentrations of DOACs. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and valida...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research