Overlap of Antibiotic Resistant Campylobacter jejuni MLST Genotypes Isolated From Humans, Broiler Products, Dairy Cattle and Wild Birds in Lithuania

Antimicrobial resistance was determined for 341 thermophilic Campylobacter jejuni isolates obtained from human clinical cases (n = 101), broiler products (n = 98), dairy cattle (n = 41) and wild birds (n = 101) with known multilocus sequence types (MLST) in Lithuania. The minimum inhibitory concentration (MIC) values for ciprofloxacin, tetracycline, gentamycin, ceftriaxone and erythromycin were determined with the agar dilution method. MIC values were compared with MLST types to find possible associations among isolation source, sequence type and resistance to antibiotics. The proportions of resistant strains were 94.2% (human), 95% (wild birds), 100% (broiler products) and 100% (dairy cattle) for one of the tested antibiotics. Most frequently, resistance to ciprofloxacin was observed (91.5%), followed by ceftriaxone with 60.4%, and tetracycline (37.8%). However only three C. jejuni strains were resistant to erythromycin (0.9%) and all tested thermophilic Campylobacter strains were sensitive to gentamicin. Most of the examined C. jejuni isolates (80.6%) showed resistance to at least one of three profiles: CIP+AXO (28.1%), TET+ CIP+AXO (26.7%) and CIP (25.8%). Statistically significant differences in resistance to tetracycline were found between C. jejuni strains obtained from cattle (85.4%) and broiler products (64.3%) (P
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research

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ConclusionOur findings argue against a co-selection mechanism of antimicrobial drug-resistance and zinc tolerance after dietary zinc supplementation in weaning piglets. An explanation for an increase in multi-drug resistant isolates from piglets with high zinc dietary feeding could be that resistant bacteria to antimicrobial agents are more persistent to stresses such as zinc or copper exposure.
Source: Gut Pathogens - Category: Microbiology Source Type: research
Mycobacteria are unique in many aspects of their biology. The development of genetic tools to identify genes critical for their growth by forward genetic analysis holds great promises to advance our understanding of their cellular, physiological and biochemical processes. Here we report the development of a novel transposon, MycoTetOP2, to aid the identification of such genes by direct transposon mutagenesis. This mariner-based transposon contains nested anhydrotetracycline (ATc)-inducible promoters to drive transcription outward from both of its ends. In addition, it includes the Escherichia coli R6Kγ origin to faci...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In conclusion, the detection of two different NDM-5 E. coli strains from a pediatric patient with a history of travel to the Far East countries strongly highlight an increasing trend and risk of importation from such areas.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
This study sheds light on the virulence and AMR potential of E. coli ST302 strains and informs AMR genomic surveillance.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Purpose of review To review recent findings regarding the control and treatment of campylobacteriosis. Recent findings The application of improved diagnostics has led to an upward shift in the attributable burden of Campylobacter infections, in both the United States and Europe as well as in resource-poor settings. Increased focus has brought a fundamental feature of campylobacteriosis -- the ability to cause relapsing disease back into focus, and expanding data on antimicrobial resistance has lead from a switch in first-line therapy for severe diarrhea from quinolones to azithromycin in most contexts, even as evidenc...
Source: Current Opinion in Infectious Diseases - Category: Infectious Diseases Tags: GASTROINTESTINAL INFECTIONS: Edited by Gagandeep Kang and Eric R. Houpt Source Type: research
Conclusion This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the viability of ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion The present results demonstrated that Chilean salmon farms play an important role as reservoirs of sulfonamide- and trimethoprim-resistant bacteria. The prevalence of conjugative plasmids and integrons among sul-carrying bacteria suggests these bacteria, mainly belonging to the Pseudomonas genus may contribute to high spread of bacterial resistance to sulfonamides and other antibacterials in environments associated with Chilean salmon farms. This is the first study reporting the occurrence of transferable sul and dfr genes and integrons among the antimicrobial resistant bacteria associated with Chilean salmonid...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, a BER homolog encoded by cj0595c (named nth) in C. jejuni was analyzed for endonuclease III activity and for its role in maintaining genomic stability. It was found that inactivation of nth resulted in elevated frequencies of spontaneous fluoroquinolone-resistant (FQR) and oxidative stress resistant (OXR) mutants, compared with the wild-type strain in C. jejuni. Sequencing analysis of the FQR and OXR mutants revealed that the elevated mutation rates were associated with C → T or G → A transition in gyrA (FQR mutants) or perR (for OXR mutants). In an in vitro assay, a purified recombinant C. jejuni ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Author Affiliations open 1Public Health Institute, Oakland, California, USA 2Department of Epidemiology, University of Michigan, Ann Arbor, Michigan, USA 3Instituto de Microbiologia, Colegio de Ciencias Biologicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador 4Department of Epidemiology and Biostatistics, Michigan State University, East Lansing, Michigan, USA 5Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA 6Department of Veterinary and Biomedical Sciences, University of Minnesota, St. Paul, Minnesota, USA PDF Version (176 KB) Abstract ...
Source: EHP Research - Category: Environmental Health Authors: Tags: Brief Communication Source Type: research
Author Affiliations open 1Center for Disease Dynamics, Economics &Policy, Washington, DC, USA 2Public Health Foundation of India, New Delhi, Delhi, India 3Dept. of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA 4School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India 5Dept. of Ecology and Evolutionary Biology, Princeton University, Princeton, New Jersey, USA 6SRL Diagnostics, FORTIS Hospital, Noida, Uttar Pradesh, India 7SRL Diagnostics, Mumbai, Maharashtra, India 8Veterinary &Animal Husbandry Extension, Guru Angad D...
Source: EHP Research - Category: Environmental Health Authors: Tags: Research Source Type: research
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