Integration analysis of a miRNA-mRNA expression in A549 cells infected with a novel H3N2 swine influenza virus and the 2009 H1N1 pandemic influenza virus.

Integration analysis of a miRNA-mRNA expression in A549 cells infected with a novel H3N2 swine influenza virus and the 2009 H1N1 pandemic influenza virus. Infect Genet Evol. 2019 Jun 14;:103922 Authors: Gao L, Gao J, Liang Y, Li R, Xiao Q, Zhang Z, Fan X Abstract Swine are reservoirs for anthropogenic/zoonotic influenza viruses, and the prevalence and repeated introduction of the 2009 H1N1 pandemic influenza virus (pdm/09) into pigs raises the possibility of generating novel swine influenza viruses with the potential to infect humans. However, studies aiming to identify miRNAs involved in the transfer of novel swine influenza virus infection to human cells are rare. In this investigation, from the view of small RNA, microarrays and high-throughput sequencing were used to detect differentially expressed miRNAs and mRNAs after human lung epithelial cells were infected with the following three stains of influenza viruses: a novel H3N2 swine influenza virus reassorted with pdm/09 fragments, pdm/09 and classical swine influenza virus. A miRNA-mRNA interaction map was generated to show the correlation between miRNAs related to infection by the viruses with human infective potential/capability. The expression of 4 miRNAs (hsa-miR-96-5p, hsa-miR-140-5p, hsa-miR-30a-3p and hsa-miR-582-5p) and 5 relevant mRNAs (RCC1, ERVFRD-1, RANBP1, SCARB2 and RPS29) was determined. The integration analysis indicated that these candidates have rarely been reported to be associat...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research

Related Links:

Authors: Pérez-García F PMID: 31326095 [PubMed - as supplied by publisher]
Source: Medicina Clinica - Category: General Medicine Tags: Med Clin (Barc) Source Type: research
[Nation] The Ministry of Health has declared Kenya free of the avian and swine flu following a scare of an outbreak of the deadly diseases over the weekend.
Source: AllAfrica News: Health and Medicine - Category: African Health Source Type: news
From the European Congress of Virology in Rotterdam, Vincent and local co-host Ben Berkhout speak with Ron Fouchier, Rosina Girones, and Marie-Paule Kieny about their careers and their work on influenza virus, environmental virology, and developing an Ebola virus vaccine during an epidemic. Click arrow to play Download TWiV 557 (41 MB .mp3, 67 min) Subscribe (free): iTunes, Google […]
Source: virology blog - Category: Virology Authors: Tags: This Week in Virology antigenic drift ebola virus environmental virology epidemic global health H2N2 influenza virus pandemic vaccine viral viruses viruses in water Source Type: blogs
(University of Tennessee at Knoxville) On average, American colleges and universities with nursing programs offer about one hour of instruction in handling catastrophic situations such as nuclear events, pandemics, or water contamination crises, according to two recent studies coauthored by a nursing professor at the University of Tennessee, Knoxville.
Source: EurekAlert! - Medicine and Health - Category: International Medicine & Public Health Source Type: news
In conclusion, the reported results highlight the importance of AIV attachment to trachea in many avian species. Finally, the importance of chickens and mallards in AIVs dynamics was illustrated by the abundant AIV attachment observed. Introduction Influenza A viruses (IAVs) are pathogens of global concern in both human and veterinary medicine (Webster et al., 1992; Stöhr, 2002; Olsen et al., 2006; Wiethoelter et al., 2015). Wild birds are well-described hosts of avian influenza viruses (AIVs) and longitudinal surveillance studies have demonstrated a plethora of low pathogenic AIVs (LPAIVs) circulating in wild...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we used a swine pH1N1 challenge virus to investigate the efficacy of whole inactivated virus vaccines homologous or heterologous to the challenge virus as well as a commercial vaccine. We found that vaccine-mediated protection was most effective when vaccine antigen and challenge virus were homologous and correlated with the specific production of neutralising antibodies and a cellular response to the challenge virus. We conclude that a conventional whole inactivated SwIV vaccine must be antigenically matched to the challenge strain to be an effective control measure. PMID: 30914224 [PubMed - as supplied by publisher]
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
AbstractPurpose of ReviewZoonotic influenza viruses are those that cross the animal-human barrier and can cause disease in humans, manifesting from minor respiratory illnesses to multiorgan dysfunction. They have also been implicated in the causation of deadly pandemics in recent history. The increasing incidence of infections caused by these viruses worldwide has necessitated focused attention to improve both diagnostic as well as treatment modalities. In this first part of a two-part review, we describe the structure of zoonotic influenza viruses, the relationship between mutation and pandemic capacity, pathogenesis of i...
Source: Current Infectious Disease Reports - Category: Infectious Diseases Source Type: research
In this study, we examined and compared haemagglutination inhibition (HI) antibody titres against previously established IAVs and pH1N1 in 908 swine sera from 88 herds, collected before and after the 2009 pandemic. While we confirmed the historic presence of the three IAVs established in European swine, we also found that 4% of the pig sera examined after 2009 had HI antibodies only against the pH1N1 virus. Our results indicate that pH1N1 is circulating in Greek pigs and stress out the importance of a vigorous virological surveillance programme.
Source: Zoonoses and Public Health - Category: Infectious Diseases Authors: Tags: Original Article Source Type: research
Abstract In 2011-2012, contemporary North American-like H3N2 swine influenza viruses (SIVs) possessing the 2009 pandemic H1N1 matrix gene (H3N2pM-like virus) were detected in domestic pigs of South Korea where H1N2 SIV strains are endemic. More recently, we isolated novel reassortant H1N2 SIVs bearing the Eurasian avian-like swine H1-like hemagglutinin and Korean swine H1N2-like neuraminidase in the internal gene backbone of the H3N2pM-like virus. In the present study, we clearly provide evidence on the genetic origins of the novel H1N2 SIVs virus through genetic and phylogenetic analyses. In vitro studies demonst...
Source: Infection, Genetics and Evolution - Category: Genetics & Stem Cells Authors: Tags: Infect Genet Evol Source Type: research
The objectives of this study were to describe the control measures implemented since October 2013 in response to this outbreak and to compare virus occurrence in piglets ‒ the subpopulation likely responsible for viral maintenance within the herd5,6 ‒ using a pre-post intervention study design. Additionally, the pattern of maternally-derived immunity in piglets sourced from the affected herd was assessed. Methods Study farm The study farm consisted of an intensive, closed-cycle swine breeding farm housing approximately 1,100 sows/gilts and 23,000 piglets located in North-East Italy. This farm supplies ~90 day-old pi...
Source: PLOS Currents Outbreaks - Category: Epidemiology Authors: Source Type: research
More News: Bird Flu | Flu Pandemic | Genetics | H1N1 | H3N2 | Influenza | Pandemics | Science | Study | Swine Flu | Zoonoses