New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration.

In conclusion, sperm with globozoospermia showed an increased susceptibility to non-enzymatic fatty acid oxidation that could be, at least in part, responsible for fertilization failure in intracytoplasmic sperm injection. The opportunity to study a peculiar sperm pathology, as globozoospermia, could provide new insights in the relationship between lipid peroxidation and male infertility. Abbreviations: PPARγ: peroxisome proliferator γ activated receptor gamma; F2-IsoPs: F2-isoprostanes; cPLA2: cytosolic phospholipase A2; ICSI: Intracytoplasmic Sperm Injection; PLCζ: phospholipase C zeta; PUFAs: polyunsaturated fatty acid; AA: arachidonic acid; PLA2: phospholipase A2; SEM: scanning electron microscopy; TEM: transmission electron microscopy; AB: aniline blue; AO: acridine orange; FA: fatty acid; 8-iso-PGF2α: 8-iso-prostaglandin F2α; WHO: World Health Organization; DAPI: 4,6-Diamidino-2-phenylindole; PBS: phosphate buffered saline; GC/NICI-MS/MS: gas chromatography/negative-ion chemical ionization tandem mass spectrometry; BHT: butylated hydroxytoluene; PGF2α-d4: tetradeuterated derivative of Prostaglandin F2α; ELISA: enzyme-linked immunosorbent assay. PMID: 31204846 [PubMed - as supplied by publisher]
Source: Systems Biology in Reproductive Medicine - Category: Reproduction Medicine Authors: Tags: Syst Biol Reprod Med Source Type: research