New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration.

New insights into sperm with total globozoospermia: Increased fatty acid oxidation and centrin1 alteration. Syst Biol Reprod Med. 2019 Jun 17;:1-10 Authors: Moretti E, Collodel G, Salvatici MC, Belmonte G, Signorini C Abstract Sperm morphology and lipid profile have a relevant role in male fertility. We explore the status of the sperm centriole, essential structure for human fertilization and the role of fatty acid oxidation in globozoospermia, a rare sperm pathology causing infertility. A case of globozoospermia was characterized by light and electron microscopy, sperm immunolocalization was performed for centrin1, 8-iso-PGF2α and peroxisome proliferator γ activated receptor gamma (PPARγ). In semen and sperm pellet, F2-isoprostanes (F2-IsoPs) levels were determined. Finally, the cytosolic phospholipase A2 (cPLA2) assessment was carried out in spermatozoa. The experiments were also performed in sperm from three fertile men. The results of electron microscopy analysis showed spermatozoa were round-headed without acrosome and with tail coiled around the nucleus characterized by immature chromatin. Centrin1 was located around the head in globozoospermic sperm, whereas, in sperm of controls, it appears as two spots. The 8-iso-PGF2α was detected around the head and in the cytoplasmic residue; PPARγ was mainly present in the cytoplasmic residue and under the head. The fluorescent signals for all molecules were different in the spe...
Source: Systems Biology in Reproductive Medicine - Category: Reproduction Medicine Authors: Tags: Syst Biol Reprod Med Source Type: research

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Michael Grynberg1,2,3,4* and Julie Labrosse2 1AP-HP, Department of Reproductive Medicine &Fertility Preservation, Hôpital Antoine Béclère, Clamart, France 2AP-HP, Department of Reproductive Medicine &Fertility Preservation, Hôpital Jean Verdier, Bondy, France 3INSERM U1185, University Paris-Sud, Le Kremlin-Bicêtre, France 4INSERM U1133, University Paris Diderot, Paris, France The management of low prognosis patients in ART represents a challenge for reproductive specialists. Different profiles and biologic characteristics have been identified among these patients. Indeed...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
Jie Cai1†, Yi Zhang1†, Yuying Wang1, Shengxian Li1, Lihua Wang1, Jun Zheng1, Yihong Jiang1, Ying Dong1, Huan Zhou1, Yaomin Hu1, Jing Ma1, Wei Liu1,2*† and Tao Tao1*† 1Division of Endocrinology and Metabolism, Department of Internal Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Pudong, China 2Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reproductive Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Pudong, China Background: Infertility and dyslipidemia are frequently present in both women...
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
ConclusionsOverall, there was no significant difference in birth defects resulting from IVF ‐ICSI with autologous or donor oocytes. The births resulting from IVF‐ICSI pregnancies did not tend to have a higher rate of birth defects a compared with natural conceptions. The differences in the prevalence of certain birth defects (cardiovascular or central nervous system) reported in IVF‐ ICSI pregnancies may be due to improved surveillance modalities and early detection in pregnancies following IVF‐ICSI. A study with larger number of sample size will give us better understanding of the prevalence of reported incidence in this study.
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research
ConclusionsOverall, there was no significant difference in birth defects resulting from IVF ‐ICSI with autologous or donor oocytes. Overall the births resulting from IVF‐ICSI pregnancies did not have a higher rate of birth defects compared to natural conceptions. The differences in the prevalence of certain birth defects (cardiovascular or central nervous system) reported in IVF‐ICSI pregnancies may be due to improved surveillance modalities and early detection in pregnancies following IVF‐ICSI. A study with larger number of sample size will give us better understanding of the prevalance of reported incidence in th...
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: Original Research Article Source Type: research
My husband and I went to a renowned mother-child hospital in the suburbs of Mumbai to seek fertility treatment since we had an issue of male factor infertility (low sperm count and morphology). As per the doctor ’s advice (which matched what we had read ourselves), we decided to go ahead with IVF-ICSI since this would the best suited for our condition since a single sperm would be injected into a single egg, thus dealing with the low sperm motility issue. The specialist told us that my husband could give sperm deposits which would be frozen and pooled along with a fresh sample for best chances of embryos developing. ...
Source: Dr.Malpani's Blog - Category: Reproduction Medicine Source Type: blogs
This study aimed to assess the incidence of epigenetic diseases with affected imprinting genes in infants or children from pregnancies obtained through IVF/ICSI compared to infants or children from pregnancies obtained spontaneously. PATIENT DATA: This is a monocentric retrospective epidemiological study based on data from a French congenital malformations registry called REMERA (Registre des Malformations en Rhône-Alpes) which exhaustively monitors all pregnancies in Rhone-Alpes region, whatever their nature of onset (spontaneous pregnancies or pregnancies from ART). This registry collects all malformations, ex...
Source: European Journal of Medical Genetics - Category: Genetics & Stem Cells Authors: Tags: Eur J Med Genet Source Type: research
Abstract IntroductionTesticular microdissection sperm extraction (MD‐TESE) combined with intracytoplasmic sperm injection (ICSI) has made biological fatherhood possible for many men with the most severe form of male infertility, non‐obstructive azoospermia. MD‐TESE was introduced in Turku in 2008, and by 2015, 100 Finnish men with non‐obstructive azoospermia have been operated on. Material and methodsThe average age of the men was 33 years at the time of surgery. Forty‐eight had a needle biopsy previously and 56% had a testicular size
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: Original Research Article Source Type: research
ConclusionsOur SRR is comparable with international results, and cumulative live birth rate similar to other ICSI indications in Finland. Physicians and specialists need to be made aware of new treatment options to enable biological fatherhood for men with non‐obstructive azoospermia.This article is protected by copyright. All rights reserved.
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: Original Research Article Source Type: research
ConclusionsWeekend work may not be needed for IVF/ICSI‐ single‐embryo transfer treatments. However, in gonadotropin releasing hormone antagonist cycles, delaying OPU more than one day may be unfavorable.This article is protected by copyright. All rights reserved.
Source: Acta Obstetricia et Gynecologica Scandinavica - Category: OBGYN Authors: Tags: Original Research Article Source Type: research
CONCLUSIONS: AFC is more sensitive than the AMH in detecting ovarian response in women with ovarian endometrioma. The individualization of GnRH antagonist protocols in subjects having endometrioma might be improved by using an AFC-tailored approach instead of AMH. PMID: 28617534 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
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