Mapping spatiotemporal microproteomics landscape in experimental model of traumatic brain injury unveils a link to Parkinson's disease.

Mapping spatiotemporal microproteomics landscape in experimental model of traumatic brain injury unveils a link to Parkinson's disease. Mol Cell Proteomics. 2019 Jun 16;: Authors: Mallah K, Quanico J, Raffo-Romero A, Cardon T, Aboulouard S, Devos D, Kobeissy F, Zibara K, Salzet M, Fournier I Abstract Traumatic brain injury (TBI) represents a major health concerns with no clinically-approved FDA drug available for therapeutic intervention. Several genomics and neuroproteomics studies have been employed to decipher the underlying pathological mechanisms involved that can serve as potential neurotherapeutic targets and unveil a possible underlying relation of TBI to other secondary neurological disorders. In this work, we present a novel high throughput systems biology approach using a spatially-resolved microproteomics platform conducted on different brain regions in an experimental rat model of moderate of controlled cortical injury (CCI) at a temporal pattern post-injury (1 day, 3 days, 7 days, and 10 days). Mapping the spatiotemporal landscape of signature markers in TBI revealed an overexpression of major protein families known to be implicated in Parkinson's disease (PD) such as GPR158, HGMB1, Synaptotagmin and Glutamate Decarboxylase in the ipsilateral substantia nigra. In silico bioinformatics docking experiments indicated the potential correlation between TBI and PD through alpha-synuclein. In an in vitro model, stimulation wit...
Source: Genomics Proteomics ... - Category: Genetics & Stem Cells Authors: Tags: Mol Cell Proteomics Source Type: research