Relationship between TNF- α, MMP-8, and MMP-9 levels in gingival crevicular fluid and the subgingival microbiota in periodontal disease

This study evaluated the composition of periodontal microbiota with the checkerboard hybridization technique and its correlation with TNF-α, MM P-8, and MMP-9 evaluated with ELISA, of 80 patients (45 healthy, and 35 with chronic periodontitis). The frequency of the 18 species evaluated was higher in patients with bone loss compared with control group. TNF-α in gingival crevicular fluid was significantly higher in bone loss group (p <  0.01); MMP-8 (p = 0.34) by MMP-9 (p <  0.05) in bone loss group obtained lower values than in control group. Positive correlation of TNF-α was obtained withAggregatibacter actinomycetemcomitans (rho  = 0.38;p <  0.01),Fusobacterium nucleatum (rho  = 0.25;p <  0.05) andPorphyromonas gingivalis (rho  = 0.26;p <  0.05); negative correlation of MMP-8 withA. actinomycetemcomitans (rho  = 0.26;p <  0.01),Capnocytophaga sputigena (rho  = 0.33;p <  0.01), andF. nucleatum (rho  = 0.21;p <  0.05); also negative correlation of MMP-9 withF. nucleatum (rho  = 0.23;p <  0.05),P. gingivalis (rho  = 0.23;p <  0.05), andTannerella forsythia (rho  = 0.26;p <  0.01). TNF-α increased due to the increase in each count ofA. actinomycetemcomitans (β = 0.57;p = 0.00). The presence ofA. actinomycetemcomitans (β = 1.88;p = 0.00),Campylobacter rectus (β = 0.78;p = 0.01),F. nucleatum (β = 0.65;p = 0.04), andP. gingival...
Source: Odontology - Category: Dentistry Source Type: research