Spatial manipulation of magnetically-responsive nanoparticle engineered human neuronal progenitor cells

Publication date: Available online 17 June 2019Source: Nanomedicine: Nanotechnology, Biology and MedicineAuthor(s): Ivan Guryanov, Ekaterina Naumenko, Svetlana Konnova, Maria Lagarkova, Sergey Kiselev, Rawil FakhrullinAbstractHere we report a detailed investigation of the interaction of neuronal progenitor cells and neurons with polyelectrolyte-stabilized magnetic iron oxide nanoparticles. Human neuronal progenitor and neurons were differentiated in vitro from fibroblast-derived induced pluripotent stem cells. The cytotoxic effects of poly(allylamine hydrochloride) was determined on human skin fibroblasts and neuronal progenitor cells. Immunocytochemical staining of lamins A/C and B in cells treated separately with poly(allylamine hydrochloride) and magnetic nanoparticles allowed to exclude these nuclear components as targets of toxic effects. We demonstrate that magnetic nanoparticles accumulated in cytoplasm and on the surface of neuronal progenitor cells neither interacted with the nuclear envelope nor penetrated into the nuclei of neuronal cells. The possibility of guidance of magnetically functionalised neuronal progenitor cells under magnetic field was demonstrated. Magnetisation of progenitor cells using poly(allylaminehydrochloride)-stabilized magnetic nanoparticles allows for successful managing their in vitro localisation in a monolayer.Graphical AbstractWe have functionalised neuronal progenitor cells and neurons with polyelectrolyte-stabilized magnetic iron oxide ...
Source: Nanomedicine: Nanotechnology, Biology and Medicine - Category: Nanotechnology Source Type: research