Transcription Factor Sp1 Regulates the Expression of Calcium Channel α2δ-1 Subunit in Neuropathic Pain

Publication date: Available online 18 June 2019Source: NeuroscienceAuthor(s): Kimberly Gómez, Alejandro Sandoval, Paulino Barragán-Iglesias, Vinicio Granados-Soto, Rodolfo Delgado-Lezama, Ricardo Felix, Ricardo González-RamírezAbstractHigh voltage-activated (HVA) Ca2+ (CaV) channels are oligomeric complexes formed by an ion-conducting main subunit (Cavα1) and at least two auxiliary subunits (Cavβ and CaVα2δ). It has been reported that the expression of CaVα2δ1 increases in the dorsal root ganglia (DRGs) of animals with mechanical allodynia, and that the transcription factor Sp1 regulates the expression of the auxiliary subunit. Hence, the main aim of this work was to investigate the role of Sp1 as a molecular determinant of the exacerbated expression of CaVα2δ-1 in the nerve ligation-induced model of mechanical allodynia. Our results show that ligation of L5/L6 spinal nerves (SNL) produced allodynia and increased the expression of Sp1 and CaVα2δ-1 in the DRGs. Interestingly, intrathecal administration of the Sp1 inhibitor mithramycin A (Mth) prevented allodynia and decreased the expression of Sp1 and CaVα2δ-1. Likewise, electrophysiological recordings showed that incubation with Mth decreased Ca2+ current density in the DRG neurons, acting mostly on HVA channels. These results suggest that L5/L6 SNL produces mechanical allodynia and increases the expression of the transcription ...
Source: Neuroscience - Category: Neuroscience Source Type: research

Related Links:

ConclusionSurgical resection of the tumor may relieve the spinal cord compression in a neglected case. Even though the neurologic function after surgery rarely returns to the functional stage due to chronic neural tissue damage.
Source: International Journal of Surgery Case Reports - Category: Surgery Source Type: research
Conclusions: Stress-exaggerated visceral mechanonociception after antral cold exposure may involve up-regulation of TRPA1 expression and function on vagal afferents. Our findings reveal a novel mechanism for abnormal gastrointestinal cold sensing in IBS. PMID: 31327223 [PubMed]
Source: Journal of Neurogastroenterology and Motility - Category: Gastroenterology Tags: J Neurogastroenterol Motil Source Type: research
Condition:   Undefined Intervention:   Behavioral: Valsalva Maneuver Sponsor:   Indonesia University Not yet recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Authors: Azadinia F, Ebrahimi-Takamjani I, Kamyab M, Asgari M, Parnianpour M Abstract The characteristics of postural sway were assessed in quiet standing under three different postural task conditions in 14 patients with nonspecific chronic low back pain and 12 healthy subjects using linear and nonlinear center of pressure parameters. The linear parameters consisted of area, the mean total velocity, sway amplitude, the SD of velocity, and the phase plane portrait. The nonlinear parameters included the Lyapunov exponent, sample entropy, and the correlation dimension. The results showed that the amount of postural s...
Source: Motor Control - Category: Neurology Tags: Motor Control Source Type: research
BACK pain is a simple fact of life for many people. The toils of everyday living can place stress on this important part of the body. There are a number of ways to ease the discomfort, one study suggests this popular indoor activity may help.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
SUMMARY OBJECTIVE: To investigate the efficacy and safety of percutaneous transforaminal endoscopic discectomy (PTED) in the treatment of lumbar spinal stenosis (LSS) combined with osteoporosis. METHODS: Eighty patients with LSS combined with osteoporosis were divided into a control and PTED group, which received conventional transforaminal lumbar interbody fusion and PTED, respectively. The surgical indications, incision visual analogue scale (VAS), lumbar and leg pain VAS, lumbar Japanese Orthopaedic Association (JOA) and Oswestry disability index (ODI) scores, bone mineral density (BMD), and adverse reactions were obser...
Source: Revista da Associacao Medica Brasileira - Category: General Medicine Source Type: research
CONCLUSION This case demonstrates that, similarly to Graves ’ disease, Hashitoxicosis can also be triggered by stressful life events.RESUMO OBJETIVO Mesmo que o estresse seja conhecido h á muito tempo como um fator provocativo para a doença de Graves, sua relação com a tireoidite de Hashimoto é mais controversa. Estudos sobre esse tema são escassos. O objetivo deste artigo é relatar um caso de Hashitoxicose induzida por estresse. RESULTADOS Aqui nós relatamos um caso de Has hitoxicose induzido por um evento psicológico estressante em uma mulher de 28 anos...
Source: Revista da Associacao Medica Brasileira - Category: General Medicine Source Type: research
SUMMARY OBJECTIVE: To investigate clinical curative effects of lumbar oblique-pulling manipulation in combination with sling-exercise-therapy training on chronic nonspecific lower back pain. METHODS: A total of 60 patients with chronic nonspecific lower back pain in the Outpatient Department were included in this study. These patients were randomly divided into two groups: the observation group and the control group. The control group adopted a single sling-exercise-therapy training three times a week, while the observation group adopted lumbar oblique-pulling manipulation in combination with manipulation treatment once a ...
Source: Revista da Associacao Medica Brasileira - Category: General Medicine Source Type: research
A recent study from the U.S. showed that the technique, which uses radio-frequency waves, is 90 per cent effective, reports the European Spine Journal
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Conclusions: The MIS-OLIF technique at the L2–L5 levels has shown encouraging early surgical outcomes with a good fusion rate and rapid recovery. While utilizing the unfamiliar oblique trajectory, the use of navigation can guide the surgeon in real time and also help in reducing the radiation exposure.
Source: Neurology India - Category: Neurology Authors: Source Type: research
More News: Back Pain | Brain | Calcium | Neurology | Neuroscience | Pain