Increased genetic diversity of methicillin-resistant Staphylococcus aureus (MRSA) isolated from companion animals

Publication date: Available online 17 June 2019Source: Veterinary MicrobiologyAuthor(s): Igor Loncaric, Sarah Lepuschitz, Werner Ruppitsch, Alan Trstan, Thomas Andreadis, Nikolaos Bouchlis, Helene Marbach, Bernhard Schauer, Michael P. Szostak, Andrea T. Feßler, Frank Künzel, Theresia Licka, Burkhard Springer, Franz Allerberger, Stefan Monecke, Ralf Ehricht, Stefan Schwarz, Joachim SpergserAbstractThe aim of the present study was to investigate the diversity of methicillin-resistant Staphylococcus aureus (MRSA) that originated from Austrian companion animals during the last five-year period. A total of 90 non-repetitive MRSA isolates were obtained during diagnostic activities from autumn 2013 to autumn 2018, originated from horses(n = 62), cats (n = 13), dogs (n = 10, rabbits (n = 2), a domestic canary, a zoo-kept hammer-headed bat (Hypsignathus monstrosus) and a semi-captive northern bald ibis (Geronticus eremita). Antimicrobial susceptibility testing was performed. All isolates were mecA-positive and mecC-negative. The isolates were genotyped by SCCmec, spa and dru typing, Multiple-Locus Variable number of tandem repeat Analyses (MLVA), S. aureus DNA microarray, and whole-genome sequencing (WGS). Eight sequence types (STs - ST398, ST5275 (new ST), ST225, ST8, ST22, ST152, ST1, and ST45), three SCCmec types (II, IV, and V), sixteen spa types (t003, t008, t011, t015, t032, t034, t1381, t1928, t1985, t223, t334, t355, t430, t6447, t6867, and t7105), fourteen d...
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research