Fluorescence polarization-based detection of cancer-related mutations using target-initiated rolling circle amplification

Analyst, 2019, Accepted Manuscript DOI: 10.1039/C9AN00429G, CommunicationWoo Young Kwon, Byung Seok Cha, Seokjoon Kim, Sung Hyun Hwang, Ji Min Kim, Kalimuth Kalishwaralal, Hyun Gyu Park, Ki Soo Park We devised a new method to detect cancer-related mutations based on target-initiated rolling circle amplification in combination with fluorescence polarization. We then applied this method to identify the presence of... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research

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Publication date: Available online 16 July 2019Source: Journal of Advanced ResearchAuthor(s): Laura Botta, Luigino Dal Maso, Stefano Guzzinati, Chiara Panato, Gemma Gatta, Annalisa Trama, Massimo Rugge, Giovanna Tagliabue, Claudia Casella, Bianca Caruso, Maria Michiara, Stefano Ferretti, Flavio Sensi, Rosario Tumino, Federica Toffolutti, Antonio Giampiero Russo, Anna Luisa Caiazzo, Lucia Mangone, Walter Mazzucco, Silvia IacovacciAbstractThe aims of this study were to provide life expectancy (LE) estimates of cancer patients at diagnosis and LE changes over time since diagnosis to describe the impact of cancer during patien...
Source: Journal of Advanced Research - Category: Research Source Type: research
Publication date: Available online 17 July 2019Source: Academic RadiologyAuthor(s): Henkjan Huisman
Source: Academic Radiology - Category: Radiology Source Type: research
ConclusionIt is feasible to use CNN based algorithm to accurately distinguish cancerous regions in OCT images. This fully automated method can overcome limitations of manual interpretation including interobserver variability and speed of interpretation and may enable real-time intraoperative margin assessment.
Source: Academic Radiology - Category: Radiology Source Type: research
In this study, a data processing pipeline for spatially resolved metabolomics analysis of tumor microregion heterogeneity was developed and built into a graphical interface with MSI software. Biological tissue sections were analysed by ambient air-flow assisted desorption electrospray ionization mass spectrometry imaging. Histology-driven and characterized ion images overlay combined with metabolic feature-based spatial segmentation were developed to accurately extract the metabolic profile from the tissue microregion of interest. In addition, appropriate data pretreatment methods were investigated to evaluate their abilit...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
Abstract In cancer diagnostics, specific analysis of blood-circulating proteins biomarkers of cancer is often complicated both by their inherently low concentrations and by strong interference from serum/blood proteins. Here, we report a simple and robust electrochemical cellulase-linked sandwich assay on magnetic beads (MBs) for fM-sensitive analysis of the Human Epidermal growth factor Receptor-2 HER-2/neu protein that is over-expressed in most aggressive breast cancers. In the assay, a sandwich is assembled by capturing HER-2/neu on either antibody (Ab) or aptamer-modified MBs accompanied by reaction with the s...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research
Publication date: September 2019Source: Journal of Functional Foods, Volume 60Author(s): Jingwen Xu, Sriram K. Vidyarthi, Weibin Bai, Zhongli PanAbstractRosa roxburghii (R. roxburghii), primarily distributed in southwest region in China, is known for its remarkable high vitamin C, SOD, and flavonoids contents. A diet rich in beneficial antioxidants such as polysaccharides, vitamin C, superoxide dismutase (SOD), and phenolics is associated with reducing risks of chronic diseases. This review examines the chemical composition in fruit of R. roxburghii and their functional properties. Recent studies showed the inhibitory effe...
Source: Journal of Functional Foods - Category: Nutrition Source Type: research
I recently attended the second Ending Age-Related Diseases conference in New York, hosted by the Life Extension Advocacy Foundation (LEAF). The mix of attendees was much the same as last year: an even split between scientists, entrepreneurs, investors, patient advocates, and interested onlookers, all focused on the treatment of aging as a medical condition. The presentations were similarly a mix of scientists talking about their research programs, entrepreneurs presenting on the data produced by their companies, and investors discussing the state of the industry. For my part, I have already presented several times t...
Source: Fight Aging! - Category: Research Authors: Tags: Healthy Life Extension Community Source Type: blogs
Darla Jaye, from The Woodlands, Texas, was diagnosed with breast cancer in February. She finished her final radiation treatment at Harris Health Systems on Monday, July 15.
Source: the Mail online | Health - Category: Consumer Health News Source Type: news
Source: BMJ News - Category: General Medicine Source Type: research
Publication date: Available online 16 July 2019Source: The Lancet HaematologyAuthor(s): Anne-Sophie Michallet, Marie-Sarah Dilhuydy, Fabien Subtil, Valerie Rouille, Beatrice Mahe, Kamel Laribi, Bruno Villemagne, Gilles Salles, Olivier Tournilhac, Alain Delmer, Christelle Portois, Brigitte Pegourie, Veronique Leblond, Cecile Tomowiak, Sophie de Guibert, Frederique Orsini, Anne Banos, Philippe Carassou, Guillaume Cartron, Luc Mathieu ForneckerSummaryBackgroundIn patients with chronic lymphocytic leukaemia, achievement of a complete response with minimal residual disease of less than 0·01% (ie,
Source: The Lancet Haematology - Category: Hematology Source Type: research
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