Contamination of yellow-feathered broiler carcasses: Microbial diversity and succession during processing.

This study investigated the effect of processing water and processing time on the microbial diversity of yellow-feathered broiler carcasses at select stages of slaughter during one commercial processing day using a high-throughput sequencing technique targeting the V3V4 region of the 16S RNA gene. Our results demonstrated that Firmicutes and Proteobacteria were the dominant bacterial phyla of broiler carcasses and processing water in the chiller tank, whereas the processing water in the scalder tank contained a high abundance of Firmicutes and Deinococcus-Thermus. At the genus level, Escherichia-Shigella and Streptococcus were present on broiler carcasses with high abundances after defeathering, but their abundance decreased after washing and chilling. The bacterial community structure was revealed to become more complex at later stages of processing, as indicated by the consistent increase in microbial alpha diversity metrics (Chao 1, Shannoneven and Shannon) throughout the processing stages (p 
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research

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In this study the effects against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacterbaumannii, Streptococcus pyogenes, Proteus vulgaris, Salmonella typhi, Shigella spp., Candida albicans, Candida parapsilosis, Anopheles stephensi, Aedes aegypti, A. aegypti, A. albopictus, Culex pipiens, Trypanosoma brucei, Leishmania major, Trichomonas. Vaginalis, poliovirus type 1, coxsackie virus B, echovirus 6, West Nile Virus, herpes virus type 1, HSV-1 virus, Fusarium spp., Aspergillus flavus, Aspergillus niger, C. albicans, Alternaria alternata, Setosphaeria turcica and Magnaporthe grisea was revealed. ...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
CONCLUSIONS: A significant dysbiosis was found in UC and CD patients compared to controls. PMID: 31293117 [PubMed - as supplied by publisher]
Source: Minerva Gastroenterologica e Dietologica - Category: Gastroenterology Tags: Minerva Gastroenterol Dietol Source Type: research
Volume 15, Issue 6, 2019, Page 1338-1356 .
Source: Human Vaccines and Immunotherapeutics - Category: Allergy & Immunology Authors: Source Type: research
Shigella species are a major cause of dysentery and may attribute for death worldwide. Currently antibiotic resistance became the critical challenges for management of infectious disease. The aim was to conduct a...
Source: Annals of Clinical Microbiology and Antimicrobials - Category: Microbiology Authors: Tags: Research Source Type: research
ConclusionOur study showed high prevalence of enteric bacterial pathogens among food handlers. All isolates were susceptible to ciprofloxacin. However, a substential number of isolates were resistant to commonly prescribed antibiotics and the prevalence of MDR was high.
Source: Antimicrobial Resistance and Infection Control - Category: Infectious Diseases Source Type: research
Publication date: 15 November 2019Source: Journal of Molecular Structure, Volume 1196Author(s): S. Jayakumar, D. Mahendiran, D. Arumai Selvan, A. Kalilur RahimanAbstractThe C-centered bis(imidazol-1-yl)methane-based heteroscorpionate metal(II) complexes of the type [M(L1−3)Cl] (1–9), where M = Mn(II), Ni(II) or Cu(II) have been synthesized using the heteroscorpionate ligands, (2-hydroxyphenyl)bis(imidazol-1-yl)methane (HL1), (4-diethylamino-2-hydroxyphenyl)bis(imidazol-1-yl)methane (HL2) and (5-bromo-2-hydroxyphenyl)bis(imidazol-1-yl)methane (HL3). The spectral and theoretical studies suggested tetrahedral ...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
Adhesive type 1 pili from enteroinvasive, Gram-negative bacteria mediate attachment to host cells. Up to 3000 copies of the main pilus subunit, FimA, assemble into the filamentous, helical quaternary structure of the pilus rod via a mechanism termed donor-strand complementation, in which the N-terminal extension of each subunit, the donor strand, is inserted into the incomplete immunoglobulin-like fold of the preceding FimA subunit. For FimA from Escherichia coli, it has been previously shown that the protein can also adopt a monomeric, self-complemented conformation in which the donor strand is inserted intramolecularly i...
Source: Journal of Biological Chemistry - Category: Chemistry Authors: Tags: Protein Structure and Folding Source Type: research
Abstract Two new phthalide derivatives, (-)-3-carboxypropyl-7-hydroxyphthalide (1) and (-)-3-carboxypropyl-7-hydroxyphthalide methyl ester (2), were isolated from the endophytic fungus Penicillium vulpinum isolated from the Chinese medicinal plant Sophora tonkinensis. Their structures were elucidated using spectroscopic methods, mainly on 1D and 2D NMR. Compound 1 exhibited medium antibacterial activities against Bacillus subtilis, Shigella dysenteriae and Enterobacter areogenes with MIC values of 12.5-25 μg/mL, and 2 showed a medium inhibition to E. areogenes with MIC value of 12.5 μg/mL. ...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
Conclusions: The carrier rate of Salmonella and Shigella was relatively low. Most isolates have developed resistance to amoxicillin, ampicillin, and tetracycline. The odds of Salmonella and Shigella was high among those who lack a habit of hand washing with soap after the use of the toilet and with untrimmed fingernails. Regular screening and appropriate hygienic control measures are needed in place to reduce the risk of infections. PMID: 31263372 [PubMed]
Source: Environmental Health Insights - Category: Environmental Health Authors: Tags: Environ Health Insights Source Type: research
Emerging evidence suggests that altered intestinal microbiota plays an important role in the pathogenesis of many liver diseases, mainly by promoting inflammation via the “intestinal microbiota-immunity-liver” axis. We aimed to investigate the fecal microbiome of liver recipients with abnormal/normal liver function using 16S rRNA gene sequencing. Fecal samples were collected from 90 liver recipients (42 with abnormal liver function (Group LT_A) and 48 with normal liver function (Group LT_N)) and 61 age- and gender-matched healthy controls (HCs). Fecal microbiomes were analyzed for comparative composition, diver...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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