A Surface Acoustic Wave (SAW) biosensor method for functional quantification of E. colil-asparaginase.

We present here a SAW biosensor based approach for the functional quantification of Escherichia colil-asparaginase (E. colil-ASNase), using polyclonal antibody (pAb) as the interaction partner immobilized on the chip. Different immobilization strategies of pAb were initially evaluated, resulting in the BS3 activated amide coupling via protein G strategy as the final immobilization method. The method was validated by evaluating the selectivity, linearity, as well as accuracy (a recovery of 102.4%) and precision (RSD of 8.5%). The application of the validated method on different samples encompassing different lots of E. colil-ASNase, deamidated E. colil-ASNase and dry-heated E. colil-ASNase (80 °C, 10 min) indicated the suitability of the developed SAW method to quantify E. colil-ASNase. We suggest this SAW method can be adopted as a pharmaceutical QC method.
PMID: 31202354 [PubMed - in process]

Source: Talanta - June 17, 2019 Category: Chemistry Authors: Yao H, Fernández CS, Xu X, Wynendaele E, De Spiegeleer B Tags: Talanta Source Type: research