Robust and Highly Specific Fluorescence Sensing of Salmonella Typhimurium Based on Dual-functional phi29 DNA Polymerase-mediated Isothermal Circular Strand Displacement Polymerization

Analyst, 2019, Accepted Manuscript DOI: 10.1039/C9AN00843H, PaperShasha Li, Su Liu, Yicheng Xu, Rufeng Zhang, Yihan Zhao, Xiaonan Qu, Yu Wang, Jiadong Huang, Jinghua Yu A simple and robust fluorescence sensing strategy has been developed for pathogenic bacteria detection by the combination of dual functionality of phi29 DNA polymerase with isothermal circular strand displacement polymerization... The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - Category: Chemistry Authors: Source Type: research

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Authors: Poonchareon K, Pulsrikarn C, Nuanmuang N, Khamai P Abstract Salmonella enterica Serotype 4,[5],12:i:-, a monophasic variant of S. Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals. BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)5-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification c...
Source: International Journal of Microbiology - Category: Microbiology Tags: Int J Microbiol Source Type: research
Publication date: Available online 18 July 2019Source: CellAuthor(s): Yue Xu, Ping Zhou, Sen Cheng, Qiuhe Lu, Kathrin Nowak, Ann-Katrin Hopp, Lin Li, Xuyan Shi, Zhiwei Zhou, Wenqing Gao, Da Li, Huabin He, Xiaoyun Liu, Jingjin Ding, Michael O. Hottiger, Feng ShaoSummaryAntibacterial autophagy (xenophagy) is an important host defense, but how it is initiated is unclear. Here, we performed a bacterial transposon screen and identified a T3SS effector SopF that potently blocked Salmonella autophagy. SopF was a general xenophagy inhibitor without affecting canonical autophagy. S. Typhimurium ΔsopF resembled S. fl...
Source: Cell - Category: Cytology Source Type: research
Two people have died following a multi-state outbreak of salmonella infections linked to backyard poultry, U.S. health officials said on Friday.
Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
AbstractNeutrophil mobilization is a crucial response to protect the host against invading microorganisms. Neutrophil recruitment and removal have to be tightly regulated to prevent uncontrolled inflammation and excessive release of their toxic content causing tissue damage and subsequent organ dysfunctions. We show here the presence of live and apoptotic neutrophils in the cytoplasm of inflamed mammary, urinary and gall bladder epithelial cells following infection withE. coli and Salmonella bacteria. The entry process commenced with adherence of transmigrated neutrophils to the apical membrane of inflamed epithelial cells...
Source: Veterinary Research - Category: Veterinary Research Source Type: research
CONCLUSIONS This work calls for continuous surveillance for the emergence of antibiotic resistance and new clones in a geographical area.
Source: Revista da Sociedade Brasileira de Medicina Tropical - Category: Tropical Medicine Source Type: research
This study provides comprehensive evidence on using Bigelow model to predict the real-time microbial reduction of bacteria in a RF process.
Source: Food Control - Category: Food Science Source Type: research
We report the crystal structure of TSP2 in complex with a repeating unit of the O157 O-antigen. We demonstrate that according to the specificity of its tailspikes TSP2, TSP3, and TSP4, CBA120 can infect E. coli O157, O77, and O78, respectively. We also show that CBA120 infects Salmonella enterica serovar Minnesota and this host range expansion is likely due to the function of TSP1. Finally, we describe the assembly pathway and the architecture of the TSP1-TSP2-TSP3-TSP4 branched complex in CBA120 and its related ViI-like phages.Graphical abstract
Source: Journal of Molecular Biology - Category: Molecular Biology Source Type: research
In this study, the roles of PBD-2 in protecting against bacterial infection were further investigated. The biochemical indexes of the blood sample, body weights, histological morphologies and weights of the organs of TG mice expressing PBD-2 were measured. Results confirmed that these mice showed normal physiological features. An assay of Salmonella Typhimurium infection was conducted on wild-type (WT) and TG mice. The TG mice possessed higher survival rate, less body weight loss and pathological changes and smaller recovery rates of bacteria after infection with Salmonella Typhimurium. The in vitro synthetic PBD-2 and the...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
AbstractNon-typhoidalSalmonella (NTS) spp. causes about 40% of all infective aortitis and it is characterized by high morbidity and mortality. Human infection occurs by fecal –oral transmission through ingestion of contaminated food, milk, or water (inter-human or zoonotic transmission). Approximately 5% of patients with NTS gastroenteritis develop bacteremia and the incidence of extra-intestinal focal infection in NTS bacteremia is about 40%. The organism can reach an extra-intestinal focus through blood dissemination, direct extension from the surrounding organs and direct bacterial inoculation (e.g. invasive medic...
Source: Infection - Category: Infectious Diseases Source Type: research
Publication date: 16 July 2019Source: Cell Reports, Volume 28, Issue 3Author(s): Dan Xiong, Li Song, Shizhong Geng, Yang Jiao, Xiaohui Zhou, Hongqin Song, Xilong Kang, Yi Zhou, Xiulong Xu, Jun Sun, Zhiming Pan, Xinan JiaoSummaryToll-like receptors (TLRs) activate innate immunity via interactions between their Toll/interleukin-1 (IL-1) receptor (TIR) domain and downstream adaptor proteins. Here we report that Salmonella Enteritidis produces a secreted protein (TcpS) that contains both a TIR domain and a coiled-coil domain. TcpS blocks MyD88- and TRIF-mediated TLR signaling, inhibits inflammatory responses, and promotes bact...
Source: Cell Reports - Category: Cytology Source Type: research
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