Highly sensitive biosensor based on graphene–poly (3-aminobenzoic acid) modified electrodes and porous-hollowed-silver-gold nanoparticle labelling for prostate cancer detection

Publication date: 1 October 2019Source: Sensors and Actuators B: Chemical, Volume 296Author(s): Chammari Pothipor, Natta Wiriyakun, Thitirat Putnin, Aroonsri Ngamaroonchote, Jaroon Jakmunee, Kontad Ounnunkad, Rawiwan Laocharoensuk, Noppadol AroonyadetAbstractRecent studies show that electrochemical biosensors offer simple, robust and low-cost solution for point-of-care applications. To meet rigorous requirements of early disease diagnosis and other medical applications, nanomaterials have been investigated to increase sensitivity and selectivity of electrochemical sensors. Herein, we successfully developed graphene-poly(3-aminobenzoic acid) (GP-P3ABA) nanomaterial-conductive polymer composite for electrode modification and metal ion loaded porous-hollowed-silver-gold core-shell nanoparticles (PHSGNPs) labelling for signal amplification. With GP-P3ABA modified electrodes, the sensing response is about 16 times higher than ones without nanocomposite due to reduction in electrode impedance and more probe binding sites from P3ABA terminals, respectively. Incubation time and volume of CuCl2 solution were optimized for maximum absorbed Cu ions on the nanoparticles and electrochemical current. It is found that our investigated system has about 3 folds higher electrochemical current from PHSGNPs than from gold nanoparticles (AuNPs) due to larger surface area of PHSGNPs to anchor Cu ions. With combination of GP-P3ABA electrode modification and PHSGNP labelling, sensing performance is ...
Source: Sensors and Actuators B: Chemical - Category: Chemistry Source Type: research

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This study aimed to explore the correlation of circular RNA itchy E3 ubiquitin protein ligase (circ-ITCH) with pathological features as well as its predictive value on prognosis in prostate cancer patients. METHODS: Three hundred and twenty-four patients with prostate cancer underwent radical prostatectomy were consecutively included in this retrospective study, and patients' specimens of tumor as well as paired adjacent tissues were collected for detection of circ-ITCH expression. Patients' baseline characteristics and survival data were obtained from follow-up records, and correlation of circ-ITCH with patients' path...
Source: Cancer Biomarkers - Category: Cancer & Oncology Tags: Cancer Biomark Source Type: research
Condition:   Prostate Cancer Interventions:   Diagnostic Test: CellSearch CTC platform;   Diagnostic Test: Parsortix CTC platform Sponsor:   Lawson Health Research Institute Recruiting
Source: ClinicalTrials.gov - Category: Research Source Type: clinical trials
Lung cancer patients undergoing radiotherapy during the time of a declared...Read more on AuntMinnie.comRelated Reading: Single-dose brachytherapy treats low-risk prostate cancer ASTRO: Prior authorization delays radiation treatment SABR plus surgery is effective for treating lung cancer Shorter radiotherapy works for prostate cancer treatment ASTRO updates guideline for lung cancer radiation therapy
Source: AuntMinnie.com Headlines - Category: Radiology Source Type: news
PROSTATE CANCER is now a bigger killer than breast cancer in the UK. The disease develops slowly over time. If this sensation is felt when urinating it could mean you are at risk. Have you ever felt this before?
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Contributors : Yuuki Imai ; Yuichiro SawadaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensProstate cancer is a high frequent disease. Early stage prostate cancer can be cured with high probability by early treatment, but hormone refractory prostate cancer and progressive prostate cancer is poor prognosis. Orphan GPCRs have the potential to become a drug discovery target in various carcinomas, but there are few reports in prostate cancer and there are still many possibilities. By big data analysis, we focused on the biological properties of the prostate cancer cell line and found GPRC...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
In conclusion, CD44 expression is significantly associated with tumor aggressiveness in prostate cancer and activation of IL-6 signaling leads to a suitable microenvironment for the induction of CD44 expression. Based on our study, reduced tumor burden was associated with attenuated IL-6 signaling and augmented tumor rejection in the microenvironment, which might mediate the benefit of clinical adoption with aggressive local therapy.
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Androgen receptor (AR) is a ligand-activated transcription factor and a key driver of prostate cancer (PCa) growth and progression. Understanding the factors influencing AR-mediated gene expression provides new opportunities for therapeutic intervention. Poly(ADP-ribose) Polymerase (PARP) is a family of enzymes, which posttranslationally modify a range of proteins and regulate many...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: PNAS Plus Source Type: research
In this study, an inhibitor-free form of KLK4 was crystallized. The crystals obtained belonged to space group P1, contained four molecules in the asymmetric unit and diffracted to 1.64   Å resolution. Interestingly, a nonstandard rotamer of the specificity-determining residue Asp189 was observed in all chains. This model will provide a useful unliganded structure for the future structure-guided design of KLK4 inhibitors.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: kallikrein-related peptidase 4 KLK4 apo structure unliganded research communications Source Type: research
The objective of this study was to compare the survival outcomes of Gleason score 3+4 and 4+3 based on data from the Surveillance, Epidemiology and End Results cancer registry database, and to investigate independent prognosis-associated factors and develop nomograms for predicting survival in Gleason score 7 prostate cancer patients. Methods A retrospective study was conducted on 69,116 cases diagnosed as prostate adenocarcinoma with Gleason score 7 between 2004 and 2009. Prognosis-associated factors were evaluated using univariate and multivariate Cox regression analysis, and a 1:1 ratio paired cohort by propensity sc...
Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
Source: Cancer Management and Research - Category: Cancer & Oncology Tags: Cancer Management and Research Source Type: research
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