Neuropeptide Y immunoreactivity in the cat claustrum: A light- and electron-microscopic investigation

Publication date: November 2014 Source:Journal of Chemical Neuroanatomy, Volumes 61–62 Author(s): D.V. Hinova-Palova , B. Landzhov , E. Dzhambazova , M. Minkov , L. Edelstein , L. Malinova , A. Paloff , W. Ovtscharoff The claustrum is a telencephalic nucleus located ventrolateral to the basal ganglia in the mammalian brain. It has an extensive reciprocal connectivity with most if not all of the cerebral cortex, in particular, primary sensory areas. However, despite renewed and growing interest amongst investigators, there remains a paucity of data concerning its peptidergic profile. The aim of the present study was to examine the presence, morphology, distribution and ultrastructure of neuropeptide Y-immunoreactive (NPY-ir) neurons and fibers in the claustrum of the cat. Ten adult healthy cats from both sexes were used. All animals received human and ethical treatment in accordance with the Principles of Laboratory Animal Care. Subjects were irreversibly anesthetized and transcardially perfused with fixative solution containing glutaraldehyde and paraformaldehyde. Brains were promptly removed, postfixed and sectioned. Slices were incubated with polyclonal anti-NPY antibodies according to the standard avidin–biotin–peroxidase complex method adopted by our Department of Anatomy, Histology and Embryology. NPY-ir neurons and fibers were found to be diffusely distributed throughout the claustrum, with no obvious topographic or functional patterning other than lar...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research