Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene.
Generating Neural Stem Cells from iPSCs with Dopaminergic Neurons Reporter Gene.
Methods Mol Biol. 2019;1919:119-128
Authors: Hong H, Daadi MM
Abstract
Genetic reporters offer attractive approaches to investigate gene expression, gene function, and spatiotemporal assessment in vitro and in vivo. Tyrosine hydroxylase (TH) is the rate-limiting enzyme for the biosynthesis of the dopamine neurotransmitter, and thus it has been used as a reliable marker for dopaminergic neurons in vitro and in vivo. Herein we describe a method for making iPSC lines with TH-green fluorescent protein reporter gene using CRISPR/Cas9 technique.
PMID: 30656625 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Hong H, Daadi MM Tags: Methods Mol Biol Source Type: research