Bioinformatics Analysis of Single-Cell RNA-Seq Raw Data from iPSC-Derived Neural Stem Cells.

Bioinformatics Analysis of Single-Cell RNA-Seq Raw Data from iPSC-Derived Neural Stem Cells.
Methods Mol Biol. 2019;1919:145-159
Authors: Kim J, Daadi MM
Abstract
This chapter describes a pipeline for basic bioinformatics analysis of single-cell sequencing data (see Chap. 10 : Single-Cell Library Preparation). Starting with raw sequencing data, we describe how to quality check samples, to create an index from a reference genome, to align the sequences to an index, and to quantify transcript abundances. The curated data sets will enable differential expression analysis, population analysis, and pathway analysis.
PMID: 30656627 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 16, 2019 Category: Molecular Biology Authors: Kim J, Daadi MM Tags: Methods Mol Biol Source Type: research