Systems-Level Immune Monitoring by Mass Cytometry.

Systems-Level Immune Monitoring by Mass Cytometry.
Methods Mol Biol. 2019;1913:33-48
Authors: Lakshmikanth T, Brodin P
Abstract
As therapies involving the modulation, stimulation, and deliberate excitation of the immune system are becoming routine, better methods for monitoring immune responses in human patients are needed. Mass cytometry allows for detailed profiling of all immune cell populations and their functional responses using a simple blood sample. When combined with appropriate computational analyses, the resolution for distinguishing desired responses from unproductive or even adverse reactions to immunotherapeutic interventions increases. Here we describe a core experimental and computational framework for global, systems-level immune monitoring by mass cytometry.
PMID: 30666597 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 16, 2019 Category: Molecular Biology Authors: Lakshmikanth T, Brodin P Tags: Methods Mol Biol Source Type: research