Measurement of Inner Bark and Leaf Osmolality.

We describe how to implement this procedure for gymnosperm as well as angiosperm trees. This method uses mechanical sap extraction with a centrifuge from inner bark and leaf samples, which have gone through a deep freeze treatment and thawing. The osmotic potential of these samples is then analyzed with a freezing point or vapor pressure osmometer. The aim of these measurements is to study the spatial and temporal dynamics of phloem function.
PMID: 31197792 [PubMed - in process]

Source: Mol Biol Cell - June 16, 2019 Category: Molecular Biology Authors: Paljakka T, Lintunen A, Salmon Y, Hölttä T Tags: Methods Mol Biol Source Type: research