An injectable cationic hydrogel electrostatically interacted with BMP2 to enhance in vivo osteogenic differentiation of human turbinate mesenchymal stem cells

Publication date: October 2019Source: Materials Science and Engineering: C, Volume 103Author(s): Mal Geum Kim, Tae Woong Kang, Joon Yeong Park, Seung Hun Park, Yun Bae Ji, Hyeon Jin Ju, Doo Yeon Kwon, Young Sik Kim, Sung Won Kim, Bong Lee, Hak Soo Choi, Hai Bang Lee, Jae Ho Kim, Bun Yeoul Lee, Byoung Hyun Min, Moon Suk KimAbstractWe have designed and characterized an injectable, electrostatically bonded, in situ–forming hydrogel system consisting of a cationic polyelectrolyte [(methoxy)polyethylene glycol-b-(poly(ε-caprolactone)-ran-poly(L-lactic acid)] (MP) copolymer derivatized with an amine group (MP-NH2) and anionic BMP2. To the best of our knowledge, there have been hardly any studies that have investigated electrostatically bonded, in situ–forming hydrogel systems consisting of MP-NH2 and BMP2, with respect to how they promote in vivo osteogenic differentiation of human turbinate mesenchymal stem cells (hTMSCs). Injectable formulations almost immediately formed an electrostatically loaded hydrogel depot containing BMP2, upon injection into mice. The hydrogel features and stability of BMP2 inside the hydrogel were significantly affected by the electrostatic attraction between BMP2 and MP-NH2. Additionally, the time BMP2 spent inside the hydrogel depot was prolonged in vivo, as evidenced by in vivo near-infrared fluorescence imaging. Biocompatibility was demonstrated by the fact that hTMSCs survived in vivo, even after 8 weeks and even though relatively few macroph...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research