Fabrication and characterization of thymol-loaded nanofiber mats as a novel antimould surface material for coating cheese surface

This study covers fabrication and characterization of thymol-loaded nanofiber (TLNs) mats and evaluation of their potential to be used as novel antimould surface coating material for limitation of mould development of Aspergillus parasiticus on kashar cheese surface. Different ratios of polyvinyl alcohol (PVA)/whey protein (WP) were electrospun to yield cylindrical and fine (⁓370 nm in diameter) polymeric nanofiber mats. The fabricated nanofibers were characterized in terms of their zeta potential, electrical conductivity, molecular, thermal and morphological properties. These results revealed that there were intermolecular interactions between thymol and functional groups of PVA and WP. The most uniform nanofiber structure with smooth beadless appearance could be observed in thymol-free nanofibers (TFNs) fabricated from 60/40 ratio of PVA/WP. The nanofiber diameter and electrical conductivity values were significantly increased by increase in PVA and WP ratios, respectively. The nanofiber mats had dispersion (−7.60 mV of ζ potential) and good thermal stability (an endothermic peak at ⁓215 °C) properties. In vitro antimould activity tests results showed that TLNs mats could show a higher efficiency against mycelial growth of A. parasiticus than free thymol. In situ test results obtained at the 7th storage day confirmed in vitro test results with respect to visual inspections on coated and uncoated cheese surface, revealing that the coat...
Source: Food Packaging and Shelf Life - Category: Food Science Source Type: research

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Abstract Together with the worldwide Washoku (traditional Japanese foods and drinks) boom, interest in sake, a traditional Japanese alcoholic drink, is increasing around the world. There are few scientific analyses and studies on the production of sake or the final product itself. We show the diversity of bacterial contaminants during sake production and investigated the effects of different ingredients on sake (for example, amino acids). The koji mold Aspergillus oryzae converts rice starch into sugars, and then, the sake yeast Saccharomyces cerevisiae converts the sugars to ethanol. Comparative studies of the ba...
Source: Current Microbiology - Category: Microbiology Authors: Tags: Curr Microbiol Source Type: research
Publication date: Available online 15 June 2019Source: Journal of Molecular LiquidsAuthor(s): Ahmad Desoky M. Mohamad, M.J.A. Abualreish, Ahmed M. Abu-DiefAbstractThe present study focused on the measurement of the solubilities and the calculation of the chemical potentials of transfer of Co(III)-hydrazone complexes in mixtures of water and ethyl alcohol (EtOH), isopropyl alcohol (i-PrOH), or tert-butyl alcohol (t-BuOH) at 298 K, using the assumption that δm μθ Ph4As+ = δm μθ BPh4− (TATB). A comparison between the chemical potentials of transfer of Co(III)-hydrazone complex ions wi...
Source: Journal of Molecular Liquids - Category: Molecular Biology Source Type: research
Publication date: Available online 7 June 2019Source: LWTAuthor(s): Sofía Sampaolesi, Raúl Ricardo Gamba, Graciela Liliana De Antoni, Ángela María León PeláezAbstractBeer is the most consumed alcoholic beverage worldwide and brewery is a growing industry. Biomass by-product of beer production is constituted by viable and non-viable flocculated yeasts which are discarded. To increase the value of this waste, the potential applications of the beer fermentation residue (BFR) as probiotic and bio-preservative were studied. Strains isolated from commercial brewing starters and BFRs were...
Source: LWT Food Science and Technology - Category: Food Science Source Type: research
Publication date: Available online 18 June 2019Source: Food ControlAuthor(s): An-Dong Gong, Fei-Yan Dong, Meng-Jun Hu, Xian-Wei Kong, Fen-Fen Wei, Shuang-Jun Gong, Yi-Mei Zhang, Jing-Bo Zhang, Ai-Bo Wu, Yu-Cai LiaoAbstractThe contamination of food with Aspergillus flavus and subsequent aflatoxins is considered as one of the most severe safety problems in the world. The application of microorganisms and the produced bio-active compounds is considered as the most promising method for controlling foodborne pathogens and mycotoxins contamination both in pre- and post-harvest. Vt-7, identified as Enterobacter asburiae, was able...
Source: Food Control - Category: Food Science Source Type: research
In conclusion, we provide an integrated picture of the metabolic and regulatory aspects of the interaction of A. fumigatus and dendritic cells during infection.
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research
In conclusion, potential biological applications of PF-SeNPs were explored by antibacterial, antifungal, antioxidant and biocompatibility assays. Materials and Methods Chemicals and Reagents Sodium selenite (98%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), Tween 80, methanol, acetate buffer, tetracycline, nystatin, rhodamine 123, caspase-3 kit, Dulbecco’s modified Eagle media (DMEM), Folin-Ciocalteu reagent, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), Dulbecco’s phosphate buffered saline pH 7.4 (DPBS) and fetal bovine serum (FBS) w...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, we examined acid protease PepA's role in shochu production. For this purpose, we constructed pepA deletion and overexpression strains, using a black koji mold Aspergillus luchuensis RIB 2604 (NBRC 4314), with the Agrobacterium-mediated transformation method. The rice koji, prepared using a pepA disruptant (ΔpepA) and pepA-overexpressing strain (OEpepA), demonstrated 1/2- and 24-fold acid protease activities compared to that prepared using the parental strain, respectively. A small-scale test of sweet potato shochu brewing indicated the mash of ΔpepA had a lower amino acid concentration, while the...
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research
Microbial Ecotoxicology of Marine Plastic Debris: A Review on Colonization and Biodegradation by the “Plastisphere” Justine Jacquin1, Jingguang Cheng1, Charlène Odobel1, Caroline Pandin1, Pascal Conan1, Mireille Pujo-Pay1, Valérie Barbe1,2, Anne-Leila Meistertzheim1,3 and Jean-François Ghiglione1* 1UMR 7621, CNRS, Laboratoire d’Océanographie Microbienne, Observatoire Océanologique de Banyuls-sur-Mer, Sorbonne Université, Banyuls-sur-Mer, France 2Génomique Métabolique, Genoscope, Institut de Biologie François Jacob, Commissariat á...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
We examined the relationship between the metabolic processes of carbon catabolite repression (CCR), nitrogen catabolite repression (NCR) and virulence in a variety of A. fumigatus clinical isolates. A considerable amount of heterogeneity with respect to the degree of CCR and NCR was observed and a positive correlation between NCR and virulence in a neutropenic mouse model of pulmonary aspergillosis (PA) was found. Isolate Afs35 was selected for further analysis and compared to the reference strain A1163, with both strains presenting the same degree of virulence in a neutropenic mouse model of PA. Afs35 metabolome analysis ...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Conclusion and Perspectives CYP51 plays a crucial role in fungal invasive growth, hyphae formation and virulence, and inhibitors targeting CYP51 have always been an important component of antifungal agents. Further researches on fungal CYP51s might set about from the following aspects: First, while the detailed crystal structures of several susceptible pathogenic fungi CYP51 have been elaborated, those of drug-resistant variants are still in hypothesis. If those structures were elucidated, targeting at common drug-resistant CYP51 protein variants could probably be promising. Second, it deserves more in-depth researches to...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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