Mesenchymal Stem Cells or Interleukin-6 Improve Episodic Memory of Mice Lacking α7 Nicotinic Acetylcholine Receptors

Publication date: Available online 14 June 2019Source: NeuroscienceAuthor(s): Olena Lykhmus, Olena Kalashnyk, Lyudmyla Koval, Larysa Voytenko, Kateryna Uspenska, Serhiy Komisarenko, Olena Deryabina, Nadia Shuvalova, Vitalii Kordium, Alina Ustymenko, Vitalii Kyryk, Maryna SkokAbstractNicotinic acetylcholine receptors of α7 subtype (α7 nAChRs) are involved in regulating cognition, inflammation and cell survival. Neuroinflammation is accompanied by the decrease of α7 nAChRs in the brain and impairment of memory. We show here that α7 −/− mice possess pro-inflammatory phenotype and demonstrate worse episodic memory compared to wild-type mice. Previously we reported that mesenchymal stem cells (MSCs) restored episodic memory of lipopolysaccharide-treated wild-type mice. The aim of this study was to examine if MSCs or their soluble factors improve memory of α7 −/− mice. The α7-specific signal (ELISA) and α7 + cells (IHC) were found in the brain of α7 −/− mice on days 7 and 14 after intravenous injection of α7 + MSCs from either human umbilical cord (hMSCs) or mouse placenta (mMSCs). The intravenously injected MSCs or intraperitoneally injected hMSCs-conditioned medium transiently improved episodic memory of α7 −/− mice and decreased cytochrome c release from their brain mitochondria under the effect of Ca2 +. Either MSCs or conditioned medium stimulated an IL-6 increase in the brain, which coincided with the improvement of...
Source: Neuroscience - Category: Neuroscience Source Type: research