Apolipoprotein E and periostin are potential biomarkers of nasal mucosal inflammation: a parallel approach of in vitro and in vivo secretomes.

Apolipoprotein E and periostin are potential biomarkers of nasal mucosal inflammation: a parallel approach of in vitro and in vivo secretomes. Am J Respir Cell Mol Biol. 2019 Jun 13;: Authors: Chung YW, Cha J, Han S, Chen Y, Gucek M, Cho HJ, Nakahira K, Choi AMK, Ryu JH, Yoon JH Abstract No previously suggested biomarkers of nasal mucosal inflammation have been practically applied in clinical fields, and nasal epithelium-derived secreted proteins as biomarkers have not specifically been investigated. The goal of this study was to identify secreted proteins that dynamically change during the differentiation from basal cells to fully differentiated cells and examine whether nasal epithelium-derived proteins can be used as biomarkers of nasal mucosal inflammation, such as chronic rhinosinusitis. To achieve this goal, we analyzed two secretomes using the isobaric tag for relative and absolute quantification (iTRAQ) technique. From in vitro secretomes, we identified the proteins altered in apical secretions of primary human nasal epithelial cells (HNECs) according to the degree of differentiation; from in vivo secretomes, we identified the increased proteins in nasal lavage (NAL) fluids obtained from patients 2 weeks after endoscopic sinus surgery for chronic sinusitis. We then used a parallel approach to identify specific biomarkers of nasal mucosal inflammation; first, we selected apolipoprotein E (APOE) as a nasal epithelial cell-deriv...
Source: Am J Respir Cell Mol... - Category: Respiratory Medicine Authors: Tags: Am J Respir Cell Mol Biol Source Type: research