Designing cellulolytic enzyme systems for biorefinery: From nature to application

Publication date: Available online 13 June 2019Source: Journal of Bioscience and BioengineeringAuthor(s): Verawat Champreda, Wuttichai Mhuantong, Hataikarn Lekakarn, Benjarat Bunterngsook, Pattanop Kanokratana, Xin-Qing Zhao, Fei Zhang, Hiroyuki Inoue, Tatsuya Fujii, Lily EurwilaichitrCellulolytic enzymes play a key role on conversion of lignocellulosic plant biomass to biofuels and biochemicals in sugar platform biorefineries. In this review, we survey composite carbohydrate-active enzymes (CAZymes) among groups of cellulolytic fungi and bacteria that exist under aerobic and anaerobic conditions. Recent advances in designing effective cellulase mixtures are described, starting from the most complex microbial consortium-based enzyme preparations, to single-origin enzymes derived from intensively studied cellulase producers such as Trichoderma reesei, Talaromyces cellulolyticus, and Penicellium funiculosum, and the simplest minimal enzyme systems comprising selected sets of mono-component enzymes tailor-made for specific lignocellulosic substrates. We provide a comprehensive update on studies in developing high-performance cellulases for biorefineries.
Source: Journal of Bioscience and Bioengineering - Category: Biomedical Science Source Type: research