Evaluation of an ELISA-based visualization microarray chip technique for the detection of veterinary antibiotics in milk

Publication date: Available online 13 June 2019Source: Food ControlAuthor(s): Bingyao Du, Fang Wen, Xiaodong Guo, Nan Zheng, Yangdong Zhang, Songli Li, Shengguo Zhao, Huimin Liu, Lu Meng, Qingbiao Xu, Ming Li, Fadi Li, Jiaqi WangAbstractAntibiotics are increasingly found in dairy products. To ensure consumer safety, rapid screening methods which are accurate and cost-effective are highly in demand. A multiplexed detection technology was tested for quantification of these four kinds of antibiotics simultaneously in milk. The visual microarray technology based on enzyme-linked immunosorbent assays was utilized in this method, which can detect multiple target molecules simultaneously. This colorimetric technology provides direct results that can be detected using common commercial microplate scanners. The limit of detection (LOD) were calculated as 3.30, 3.39, 2.42 and 4.88 μg kg−1 for quinolones, tetracycline, lincomycin and streptomycin, respectively. The limits of quantity (LOQ) were defined as 8.97, 8.43, 5.36 and 10.97 μg kg−1 for quinolones, tetracycline, lincomycin and streptomycin, respectively. The accuracy of method was also adequate for these four substances with the recovery rates ranging from 77.6% to 116.4% were obtained for the detection of practical samples. According to our evaluation, this methodology can be used for rapid screening of antibiotics in internal quality control programs in dairy industry.

Source: Food Control - June 13, 2019 Category: Food Science Source Type: research