Cross-resistance profiles of malaria mosquito P450s associated with pyrethroid resistance against WHO insecticides

Publication date: Available online 13 June 2019Source: Pesticide Biochemistry and PhysiologyAuthor(s): Cristina Yunta, Kay Hemmings, Bradley Stevenson, Lizette L. Koekemoer, Tonderi Matambo, Pat Pignatelli, Michael Voice, Szilárd Nász, Mark J.I. PaineAbstractExtensive use of pyrethroids for malaria control in Africa has led to widespread pyrethroid resistance in the two major African vectors of malaria An. gambiae and An. funestus. This is often associated with constitutively elevated levels of cytochrome P450s involved with pyrethroid metabolism and detoxification. P450s have the capacity to metabolise diverse substrates, which raises concerns about their potential to cause cross-resistance. A bank of seven recombinant P450s from An. gambiae (CYPs 6M2, 6P2, 6P3, 6P4, 6P5, 9J5) and An. funestus (CYP6P9a) commonly associated with pyrethroid resistance were screened against twelve insecticides representing the five major classes of insecticides recommended by WHO for malaria control; permethrin, etofenprox and bifenthrin (type I pyrethroids), deltamethrin, lambda cyhalothrin and cypermethrin (type II pyrethroids), DDT (organochlorine), bendiocarb (carbamate), malathion, pirimiphos methyl and fenitrothion (organophosphates) and pyriproxyfen (juvenile hormone analogue). DDT was not metabolised by the P450 panel, while bendiocarb was only metabolised by CYP6P3. Pyrethroids and pyriproxyfen were largely susceptible to metabolism by the P450 panel, as were organophosphates, which ...

Source: Pesticide Biochemistry and Physiology - June 13, 2019 Category: Biochemistry Source Type: research