DGGR-lipase for effective diagnosis of pancreatitis in horses

AbstractEquine pancreatitis has no reliable ante-mortem test and is considered rare. The 1,2-o-dilauryl-rac-glycero-3-glutarate-(6 ′-methylresorufin) ester (DGGR) lipase assay is specific for pancreatic lipase (PL). It was developed for humans and validated in dogs, cats and rats as specific for pancreatitis diagnosis. We used a novel validation strategy to confirm this tissue specificity extended to horses and determine refe rence ranges and pancreatitis prevalence. One hundred thirty-nine horses were used. Biopsies were collected from 3 healthy cadavers and venous blood from 109 clinical cases and 27 training racehorses. DGGR-lipase was demonstrated to only occur significantly in the pancreas using 7 different tissues collected at euthanasia that were cryopreserved and assayed for PL and 6, other, routine biomarkers (amylase, CK, GLD, AST, ALT, ALP). PL range was determined in healthy horses and in pancreatitis cases. PL is an inexpensive, highly-effective biomarker with the highest tissue activity and specificit y of routine biomarkers. Equine pancreatitis occurs with a high prevalence and severity similar to that in dogs and cats, and in association with a wide variety of gastrointestinal disorders and with training racehorses. PL should be routinely assayed in horses with gastrointestinal disorders and mo nitored in training racehorses.
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research