Characterization of the stimulators of protein-directed ribosomal frameshifting in Theiler's murine encephalomyelitis virus.

We present an extensive mutational analysis of the frameshift stimulators (mRNA signals and 2A protein) analysing activity in in vitro translation, electrophoretic mobility shift and in vitro ribosome pausing assays. We also investigate the PRF mRNA signal with RNA structure probing. Our results substantially extend previous characterization of protein-stimulated PRF.
PMID: 31180502 [PubMed - as supplied by publisher]

Source: Cell Research - June 9, 2019 Category: Cytology Authors: Napthine S, Bell S, Hill CH, Brierley I, Firth AE Tags: Nucleic Acids Res Source Type: research