Statistical Optimization of The Four Key Factors on β-Carotene Production by Dunaliella salina ...

During recent years, there was growing demand in using microalga valuable products such asβ-carotene in health care.β-Carotene has anti-cancer and anti-aging properties for human. In Dunaliella salina cells,β-carotene has a major protecting role for biomolecules, when the production of reactive oxygen species elevated. In the present study, we investigated the influence of the four most effective factors (light intensity, temperature, nitrate and salinity concentration) and their interactions on theβ-carotene production and the total chlorophyll/β-carotene ratio in low light adapted D. salina cells. Box-Benken design and response surface methodology (RSM) was used for this purpose and optimization of the factor levels. Two models were developed to explain howβ-carotene productivity and the total chlorophyll/β-carotene ratio depend on the stress factors. Among the four stress variables forβ-carotene production, light intensity was stronger than the others. Meanwhile, interaction between light intensity and salt concentration exhibited the most important effect on the total chlorophyll/β-carotene ratio. The predicted optimal conditions for maximumβ-carotene productivity and minimum total chlorophyll/β-carotene ratio were derived from the fitted model in 200µmol photons m-2s-1 light intensity, 25ºC, 0.9 mM nitrate and 3.8 M NaCl. When the predicted condition was tested experimentally, the close results were captur...
Source: Iranian Journal of Pharmaceutical Research - Category: Drugs & Pharmacology Source Type: research