microRNA-126 Is a Tumor Suppressor of Granulosa Cell Tumor Mediated by Its Host Gene EGFL7
MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in gene regulation at post-transcriptional level. In the current study, we examined the effect of miR-126 on granulosa cell tumor (GCT), a subtype of ovarian cancer. miR-126 expression was repressed in tissues from malignant GCT patients. We showed that miR-126 could inhibit cell proliferation, migration/invasion, ovarian hormone production and promote anticancer drug-induced apoptosis in vitro. The ‘tumor suppressor’ role of miR-126 was further validated in an mice GCT model in vivo. By RNA-seq, immunohistochemical staining (IHC), Western blot and luciferase reporter assay, we identified and confirmed EGFL7 as a direct functional target of miR-126 in cancer GCs. Furthermore, we found AKT signaling pathway was associated with miR-126 and EGFL7 in cancer GCs. Taken together, our results demonstrate a function of miR-126 in the repression of GCT development via regulating EGFL7.
Sumaira Qayoom, Malti Kumari, Sameer Gupta, Madhumati GoelJournal of Cancer Research and Therapeutics 2019 15(5):1186-1188 Breast cancer is one of the main sources of ovarian metastasis. Diffuse sheet of lobular carcinoma cells can strongly mimic granulosa cell tumor (GCT) which itself is a rare ovarian neoplasm constituting <5% of all the ovarian neoplasms. A 55-year-old female presented with a painful lump in the right breast associated with nipple discharge for 4 months, which on radiological and cytological findings was suspicious of an epithelial malignancy. During routine work-up, a solid-cystic lesion in th...
ConclusionThis study demonstrates that rAMH is bioactive in the ovary for a limited time, and that pharmacological administration of rAMH during chemotherapy treatment reduces follicle activation and primordial follicle loss and significantly improves reproductive outcomes in a mouse model, and does not interfere with the therapeutic actions of the treatment. Further investigation is necessary to determine whether it has similar protective effects in the human ovary.
Abstract OBJECTIVE: About 30% of Adult type granulosa cell tumors of the ovary (AGCTs) are diagnosed in fertile age. In stage I, conservative surgery (fertility-sparing surgery, FSS), either unilateral salpingo-oophorectomy (USO) or cystectomy are possible options. The aim of this study is to compare oncological outcomes of FSS and radical surgery (RS) in apparently stage I AGCTs treated within the MITO group (Multicenter Italian Trials in Ovarian cancer). METHODS: Survival curves were calculated using the Kaplan-Meier method and compared with log-rank test. The role of clinicopathological variables as progno...
CONCLUSIONS: While no direct antitumor efficacy was documented, potentially clinically meaningful dose-related metabolic effects, including treatment of cancer cachexia, were observed that support further exploration of Activin A inhibitors that limit BMP9 blockade. PMID: 31068369 [PubMed - as supplied by publisher]
Publication date: Available online 17 April 2019Source: Gynecologic Oncology ReportsAuthor(s): Dimitrios Nasioudis, Emily M. Ko, Ashley F. Haggerty, Robert Giuntoli, Robert Burger, Mark A. Morgan, Nawar A. LatifAbstractObjectiveThe aim of the present study is to investigate the patterns of use and prognostic significance of adjuvant chemotherapy (CT) for patients with stage IC ovarian granulosa cell tumors (GCTs).MethodsWe identified patients with stage IC GCTs diagnosed between 2004 and 2015 in the National Cancer Data Base (NCDB). Logistic regression was performed to identify variables independently associated with chemo...
This study also allowed us to put forward new direct and indirect targets of the kinase. Indeed, a series of proteins involved in intracellular transport and mitochondrial physiology were significantly affected by Akt1depletion. Using in silicoanalyses, we also propose a set of kinases and transcription factors that can mediate the action of AKT1 on the deregulated transcripts and proteins. Taken altogether, our results provide a resource of direct and indirect AKT1 targets in granulosa cells and may help understand its roles in this ovarian cell type. PMID: 30992313 [PubMed - as supplied by publisher]
Introduction: Ovarian masses represent the most frequent tumors of the female genital tract in children and adolescents, about a half of them are neoplastic. A quarter of all tumors is malignant, additionally 2 –5% of those accounts for granulosa cell tumor (GCT). Despite the fact that childhood ovarian cancer is a very rare pathology and has a better prognosis due to its non-epithelial origin, the early diagnosis and specific management is necessary.
Introduction: Granulosa cell tumors (GTC) are characterized for late recurrence which happens in 10-33% of patients. Prognostic factors related with recurrence are stage at presentation, patient's age, tumor size, extent of surgery, cell atypia and mitosis.
CONCLUSIONS: Adjuvant CT was associated with improved survival for patients with advanced stage non-GC SCSTs. No clear benefit was found for those with early stage disease. PMID: 30740951 [PubMed - as supplied by publisher]
ConclusionsA somatic missense mutation in the FOXL2 gene is a sensitive molecular marker for AGCT. Mutation analysis can help distinguish malignant from benign pathology to provide appropriate treatment and disease surveillance.