Multi-platform Approach for Microbial Biomarker Identification Using Borrelia burgdorferi as a Model

The identification of microbial biomarkers is critical for the diagnosis of a disease early during infection. However, the identification of reliable biomarkers is often hampered by a low concentration of microbes or biomarkers within host fluids or tissues. We have outlined a multi-platform strategy to assess microbial biomarkers that can be consistently detected in host samples, using Borrelia burgdorferi, the causative agent of Lyme disease, as an example. Key aspects of the strategy include the selection of a macaque model of human disease, In vivo Microbial Antigen Discovery (InMAD), and proteomic methods that include microbial biomarker enrichment within samples to identify secreted proteins circulating during infection. Using the described strategy, we have identified 6 biomarkers from multiple samples. In addition, the temporal antibody response to select bacterial antigens was mapped. By integrating biomarkers identified from early infection with temporal patterns of expression, the described platform allows for the data driven selection of diagnostic targets.
Source: Frontiers in cellular and infection microbiology - Category: Microbiology Source Type: research

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The American Lyme Disease Foundation is dedicated to the prevention, diagnosis, and treatment of Lyme disease which, in the United States, has been defined historically as a tick-borne bacterial infection caused by the spirochete, Borrelia burgdorferi sensu stricto. Through its website, the Foundation plays a major role in providing reliable and scientifically accurate information to the public, medical community and various public health agencies about Lyme disease and its impact on human health.
Source: The American Journal of Medicine - Category: General Medicine Authors: Tags: COMMENTARY Source Type: research
Vector-Borne and Zoonotic Diseases, Ahead of Print.
Source: Vector-Borne and Zoonotic Diseases - Category: Infectious Diseases Authors: Source Type: research
This study demonstrates the effect of only simultaneous infection by B. burgdorferi and B. microti on each pathogen, immune response and on disease manifestations with respect to infection by the spirochete and the parasite. In our future studies, we will examine the overall effects of sequential infection by these pathogens on host immune responses and disease outcomes.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 3 July 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Sergio A. Zanzani, Sara G. Rimoldi, MariaTeresa Manfredi, Romualdo Grande, Alessia L. Gazzonis, Stefania Merli, Emanuela Olivieri, Vania Giacomet, Spinello Antinori, Giuliana Cislaghi, Giovanna Bestetti, Katiuscia Nan, Vittorio Sala, Maria R. Gismondo, Chiara Atzori, Eleonora De FaveriAbstractLyme borreliosis cases have been reported from Lombardy in northern Italy, where Ixodes ricinus is the main vector of Borrelia burgdorferi sensu lato. However, spatial and temporal variation in the incidence of Lyme borreliosis is not well un...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Date: Thursday, 08 01, 2019; Speaker: Mollie W. Jewett, Ph.D., Associate Professor, Division Head of Immunity and Pathogenesis, University of Central Florida College of Medicine; 33/1N09
Source: NIH Calendar of Events - Category: American Health Source Type: events
LYME DISEASE is an infectious disease caused by a bacterium named Borrelia, spread by ticks. It can easily be caught and having a feeling in your head could mean you have the disease.
Source: Daily Express - Health - Category: Consumer Health News Source Type: news
Publication date: Available online 3 July 2019Source: Ticks and Tick-borne DiseasesAuthor(s): Sergio A. Zanzani, Sara G. Rimoldi, MariaTeresa Manfredi, Romualdo Grande, Alessia L. Gazzonis, Stefania Merli, Emanuela Olivieri, Vania Giacomet, Spinello Antinori, Giuliana Cislaghi, Giovanna Bestetti, Katiuscia Nan, Vittorio Sala, Maria R. Gismondo, Chiara Atzori, Eleonora De FaveriAbstractLyme borreliosis cases have been reported from Lombardy in northern Italy, where Ixodes ricinus is the main vector of Borrelia burgdorferi sensu lato. However, spatial and temporal variation in the incidence of Lyme borreliosis is not well un...
Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Publication date: Available online 1 July 2019Source: Protein Expression and PurificationAuthor(s): G. Brown, A.H. Broxham, S.E. Cherrington, D.C. Thomas, A. Dyer, L. Stejskal, R.J. BinghamAbstractBorrelia are microaerophilic spirochetes capable of causing multisystemic diseases such as Lyme disease and Relapsing Fever. The ubiquitous Fe/Mn-dependent superoxide dismutase (SOD) provides essential protection from oxidative damage by the superoxide anion. Borrelia possess a single SOD enzyme - SodA that is essential for virulence, providing protection against host-derived reactive oxygen species (ROS). Here we present a metho...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
Lyme disease is a multisystem disorder caused by the spirochete Borrelia burgdorferi. A common late-stage complication of this disease is oligoarticular arthritis, often involving the knee. In ∼10% of cases, arthritis persists after appropriate antibiotic treatment, leading to a proliferative synovitis typical of chronic inflammatory arthritides. Here, we provide evidence...
Source: Proceedings of the National Academy of Sciences - Category: Science Authors: Tags: PNAS Plus Source Type: research
We describe a 20-year-old woman with left seventh nerve palsy secondary to latent syphilis of unknown duration, with complete recovery after penicillin G benzathine. In addition to reactive rapid plasma reagin and positive T. pallidum particle agglutination testing, Lyme enzyme-linked immunosorbent assay was equivocal, and Lyme Western blot was positive for immunoglobulin M. In syphilis, Lyme enzyme-linked immunosorbent assay may be falsely positive, but Western blot is known to remain negative. Similarly, in LD, fluorescent treponemal antibody absorption testing may be falsely positive, but rapid plasma reagin remains non...
Source: Infectious Diseases in Clinical Practice - Category: Infectious Diseases Tags: Case Reports Source Type: research
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