Functional genetic analysis of the leucinostatin biosynthesis transcription regulator lcsL in Purpureocillium lilacinum using CRISPR-Cas9 technology.

In this study, the CRISPR-Cas9 system was introduced to increase the efficiency of homologous recombination for the disruption of lcsL. The expression of genes in the cluster was significantly reduced in lcsL disruption mutants, and the output of leucinostatins was decreased to undetectable levels. In the lcsL overexpression strain, the expression of genes in the cluster and the yield of leucinostatins were all increased. The antagonism of both the wild type and mutant against Phytophthora infestans was also consistent with the gene expression and the output of leucinostatins. These results indicate that the gene lcsL is crucial for the regulating the synthesis of leucinostatins. PMID: 31175427 [PubMed - as supplied by publisher]

https://www.ncbi.nlm.nih.gov/pubmed/31175427?dopt=Abstract

Source: Applied Microbiology and Biotechnology - June 6, 2019 Category: Microbiology Authors: Jiao Y, Li Y, Li Y, Cao H, Mao Z, Ling J, Yang Y, Xie B Tags: Appl Microbiol Biotechnol Source Type: research