Embryonic Chimeras with Human Pluripotent Stem Cells.

We describe how to generate interspecies primate-mouse chimeras at the blastocyst and postimplantation embryo stages. Systematic evaluation of the chimeric competency of human and primate PS cells will aid in efforts to overcome species barriers and achieve higher grade chimerism in postimplantation conceptuses that could enable organ-specific enrichment of human xenogeneic PS cell derivatives in large animals such as pigs and sheep.
PMID: 31175650 [PubMed - in process]

Source: Mol Biol Cell - June 10, 2019 Category: Molecular Biology Authors: De Los Angeles A, Sakurai M, Wu J Tags: Methods Mol Biol Source Type: research