Genome sequencing and characterization of three Bacillus cereus -specific phages, DK1, DK2, and DK3

AbstractIn the study, threeBacillus cereus-specific phages, named DK1, DK2 and DK3, belonging to the familyPodoviridae, were isolated from Pearl River water and sludge in Guangzhou, China. The genomes of DK1, DK2 and DK3 were 27,180  bp, 26,357 bp, and 26,865 bp in length and contained 49, 45 and 46 open reading frames, respectively. Among the three phages, DK2 shared the highest genome sequence similarity (96% identity) with DK3. Genes encoding rRNA, tRNA, virulence factors and antibiotic resistance were absent in these pha ge genomes. In addition, comparative genomic and phylogenetic analysis revealed that they were novel phages ofB. cereus. Each genome encoded a putative endolysin that might be of value for the control of the foodborne pathogenB. cereus.
Source: Archives of Virology - Category: Virology Source Type: research