Evaluation of analytical factors associated with targeted MEFV gene sequencing using long-range PCR/massively parallel sequencing of whole blood DNA for molecular diagnosis of Familial Mediterranean fever.

CONCLUSIONS: Various analytical factors were evaluated, and the pipeline of targeted gene sequencing using LR-PCR and MPS was established. These data can enable the optimization of targeted gene sequencing using LR-PCR and MPS in the clinical laboratory.
PMID: 31173732 [PubMed - as supplied by publisher]

Source: International Journal of Clinical Chemistry - June 3, 2019 Category: Chemistry Authors: Ishige T, Itoga S, Kawasaki K, Utsuno E, Beppu M, Sawai S, Nishimura M, Ichikawa T, Nomura F, Matsushita K Tags: Clin Chim Acta Source Type: research