Evaluation of Lapatinib Cytotoxicity and Genotoxicity on MDA-MB-231 Breast Cancer Cell line

In conclusion, lapatinib induced cytotoxic and genotoxic effects on MDA-MB-231 cell line. However, laptinib reduced the EGFR protein expression and EGFR signals, it raised the apoptotic cells and TP53 gene signals, which triggered extensive DNA damage. Therefore, lapatinib is an effective TKI in triple negative breast cancer cells as elucidated by its mode of cell death.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research

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Authors: Yuan S, Luan X, Han G, Guo K, Wang S, Zhang X Abstract It is well established that long intergenic non-protein coding RNA 1638 (LINC01638) promotes the development and progression of breast cancer, whereas its roles in other human diseases are currently unknown. In the present study, expression of LINC01638 and ROCK2 was analyzed using quantitative PCR, ELISA and western blot. Receiver operating characteristic curve was used for diagnostic analysis. Cell transfections were performed to analyze interactions between LINC01638 and ROCK2, while Transwell assays were performed to analyze invasion and migration ...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
We have previously demonstrated the pivotal role of Jnk-mediated Irf-3/c-Jun in regulating nuclear factor kappa-Β ligand (RANKL)-induced osteoclastogenesis. Here, we demonstrated that proanthocyanidins (PACs) target Irf-3 to alleviate breast cancer-induced activation of osteoclasts. We also found that PACs induced apoptosis of osteoclast precursors by upregulating the ratio of bax/bcl-2 and activating caspase-3 activity. Such bone protective effect also could be observed in a bone metastasis model of breast cancer. These findings provided a novel therapeutic intervention targeting abnormal bone metabolism to alleviate...
Source: Anti-Cancer Drugs - Category: Cancer & Oncology Tags: Preclinical Reports Source Type: research
Triple negative breast cancer (TNBC) has very poor prognosis and no efficacious therapeutic options due to the absence of a validated molecular target. Therefore, novel therapeutic strategies against TNBC are urgently needed. Our team synthesized and screened a series of compounds derived from Rhein, of which 4F was selected for further analysis based on its ability to produce the vacuolated appearance of cells. Using Cell counting kit-8 assay, colony-formation assay, cell apoptosis and cell cycle assay, we compared the antitumor effects of 4F, Rhein and Cisplatin on a TNBC cell line MDA-MB-231 in vitro. The vacuoles in MD...
Source: Anti-Cancer Drugs - Category: Cancer & Oncology Tags: Case Reports Source Type: research
Conclusion: Temperature and thermal dose during HT had significant influence on complete response, duration of local control, overall survival and thermal toxicity of patients with recurrent breast cancer treated with RT + HT. Higher temperature and thermal dose improved outcome, while higher maximum temperature increased incidence of thermal toxicity. PMID: 31621437 [PubMed - in process]
Source: International Journal of Hyperthermia - Category: Internal Medicine Tags: Int J Hyperthermia Source Type: research
Publication date: January 2020Source: Radiation Physics and Chemistry, Volume 166Author(s): Fang-Yu Liu, Zhi-Wei Dong, Hui-Bin Yang, Hong-Yun ShiAbstractEven with advanced inverse intensity modulated radiation therapy (IMRT) technique, the optimization of treatment plans remains a time-consuming task and the outcome is highly influenced by the experience of the planner and time pressure. Automated treatment planning is a current hot topic and new frontier in radiotherapy, which is expected to minimize the above problems. The quality and efficacy of the IMRT plans generated by the Auto-Planning module of the Pinnacle3 treat...
Source: Radiation Physics and Chemistry - Category: Physics Source Type: research
Authors: Ma JW, Hung CM, Lin YC, Ho CT, Kao JY, Way TD Abstract [This corrects the article DOI: 10.18632/oncotarget.10410.]. PMID: 31620249 [PubMed - in process]
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Authors: Matsui A, Murata Y, Masuda N, Mori K, Takahashi M, Yamashiro K, Aogi K, Maeda S, Itou M, Ozaki S, Kuraoka K, Satou Y, Ichihara S, Tokunaga E, Taguchi K, Watanabe T, Suzuki H, Nagayama A, Nishimura R Abstract Hormone receptor and human epidermal growth factor receptor 2 (HER2) protein tests in metastatic breast cancer tissue are recommended in the guidelines of the American Society of Clinical Oncology/American Pathology Association. As part of a multi-institutional study by the National Hospital Organization, we conducted an investigation to examine these molecular markers, using cytological specimens as a...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research
Publication date: Available online 17 October 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Yanbo Yang, Jianrong Wu, David H. Bremner, Shiwei Niu, Yu Li, Xuejing Zhang, Xiaotian Xie, Li-Min ZhuAbstractSynergistic tumor treatment has recently attracted more and more attention due to its remarkable therapeutic effect. Herein, a multifunctional drug delivery system based on hyaluronic acid (HA) targeted dual stimulation responsive MoS2 nanosheets (HA-PEI-LA-MoS2-PEG, HPMP) for active interaction with CD44 receptor positive MCF-7 cells is reported. Melanin (Mel), a new type of photothermal agent and doxorubicin ...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Publication date: Available online 17 October 2019Source: Meta GeneAuthor(s): Esra BozgeyikAbstractAlthough there are several studies, biological function and therapeutic potential of miR-17-5p in breast cancer carcinogenesis remains muchly elusive. Specifically, its interaction with several cancer-associated genes remains unexplored in breast cancer. Here, we aimed to demonstrate the biological function and therapeutic potential of miR-17-5p in breast cancer through determination of the interactions between cancer-associated genes and miR-17-5p. MFC-7 breast cancer cells used in the study and cells were transfected with m...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research
Three medical stories that we didn't cover, explained.Medscape
Source: Medscape Internal Medicine Headlines - Category: Internal Medicine Tags: Family Medicine/Primary Care Article Source Type: news
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