Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal.

Proteomic analysis of male rat nucleus accumbens, dorsal hippocampus and amygdala on conditioned place aversion induced by morphine withdrawal. Behav Brain Res. 2019 Jun 04;:112008 Authors: Guo LB, Yu C, Ling QL, Fu Y, Wang YJ, Liu JG Abstract Addiction is characterized by compulsive drug seeking and taking behavior, which is thought to result from persistent neuroadaptations, encoded by changes of gene expression. We previously demonstrated that the changes in synaptic plasticity were required for the formation of aversive memories associated with morphine withdrawal. However, the proteins involved in synaptic plasticity and aversive memory formation have not been well explored. In the present study, we employed a two-dimensional gel electrophoresis (2-DE)-based proteomic technique to detect the changes of protein expression in the nucleus accumbens, amygdala and dorsal hippocampus of the rats that had developed conditioned morphine withdrawal. We found that twenty-three proteins were significantly altered in the amygdala and dorsal hippocampus after conditioned morphine withdrawal. These proteins can be classified into multiple categories, such as energy metabolism, signal transduction, synaptic transmission, cytoskeletal proteins, chaperones, and protein metabolism according to their biological functions. Eight proteins related to synaptic plasticity were further confirmed by western blot analysis. It is very likely that these identified proteins may ...
Source: Behavioural Brain Research - Category: Neurology Authors: Tags: Behav Brain Res Source Type: research

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Authors: Sheldon RR, Weiss JB, Do WS, Forte DM, Carter PL, Eckert MJ, Sohn VY Abstract INTRODUCTION: Surgery is a known gateway to opioid use that may result in long-term morbidity. Given the paucity of evidence regarding the appropriate amount of postoperative opioid analgesia and variable prescribing education, we investigated prescribing habits before and after institution of a multimodal postoperative pain management protocol. MATERIALS AND METHODS: Laparoscopic appendectomies, laparoscopic cholecystectomies, inguinal hernia repairs, and umbilical hernia repairs performed at a tertiary military medical cent...
Source: Military Medicine - Category: International Medicine & Public Health Tags: Mil Med Source Type: research
Publication date: Available online 17 October 2019Source: Physiology &BehaviorAuthor(s): Janelle A Skinner, Manohar L Garg, Christopher V Dayas, Tracy L BurrowsABSTRACTThe neuropeptide oxytocin is best known for its role during parturition and the milk-let down reflex. Recent evidence identifies a role for oxytocin in eating behaviour. After oxytocin administration, caloric intake is reduced with stronger inhibitory effects in individuals with obesity. Whether the experience of visual food cues affects secretion or circulating levels of oxytocin is unknown. This pilot study had three aims: 1) to measure fasting appetit...
Source: Physiology and Behavior - Category: Physiology Source Type: research
AbstractHealthcare environments are not exempt from the impact of stigma against mental illness and addiction, which contributes to barriers to client access and appropriate treatment. To address this concern, healthcare organizations have a growing interest in mental illness and addiction anti-stigma anti-discrimination programming as part of their staff-wide professional development. Though standard interventions demonstrate effectiveness in the short and mid-term, the evidence for long-term change is inconclusive. A flexible, innovative intervention was developed in collaboration with community health care centres to re...
Source: Journal of Community Health - Category: International Medicine & Public Health Source Type: research
Publication date: Available online 17 October 2019Source: Progress in Neuro-Psychopharmacology and Biological PsychiatryAuthor(s): Di Wen, Rongji Hui, Yi Liu, Yixiao Luo, Jian Wang, Xi Shen, Bing Xie, Feng Yu, Bin Cong, Chunling MaAbstractMethamphetamine (METH) is one of the most prevalently used illegal psychostimulants in many countries. Continuous exposure to METH leads to behavioral sensitization in animals, which can be used as a behavioral model with many mechanisms in common with relapse in humans. Molecular hydrogen has recently gained attention for its potential as a novel healthcare product with preventive and th...
Source: Progress in Neuro Psychopharmacology and Biological Psychiatry - Category: Psychiatry Source Type: research
In this study, we examined the role of D1-like dopamine receptor (D1 DR) on spatial memory in the offspring of morphine-abstinent rats. Adult male and female rats received morphine orally for 21 days and were drug-free for ten days. The rats were prepared to mate and the offspring were divided into four groups: offspring of drug-naïve parents, offspring of maternal morphine-exposed, offspring of paternal morphine-exposed, and PME + MME group. Saline or SCH23390 was injected into the hippocampus and prefrontal (PFC), and the Morris Water Maze task was performed. Afterward, the rats were sacrificed, and ph...
Source: Neuroscience Research - Category: Neuroscience Source Type: research
FRIDAY, Oct. 18, 2019 -- Electronic cigarette use in England is positively associated with overall cigarette quit rates and quit success rates, according to a study published online Oct. 17 in Addiction. Emma Beard, Ph.D., from University College...
Source: Drugs.com - Pharma News - Category: Pharmaceuticals Source Type: news
Funding Opportunity PAR-20-035 from the NIH Guide for Grants and Contracts. This Funding Opportunity Announcement (FOA) is supported by Collaborative Research on Addiction (CRAN) at the National Institutes of Health (NIH), a trans-NIH partnership composed of the National Institute on Alcohol Abuse and Alcoholism (NIAAA), the National Institute on Drug Abuse (NIDA), and the National Cancer Institute (NCI). The intent of this FOA is two-fold: (1) characterize how the neurobiological alterations, associated behaviors, and public health consequences arising from polysubstance use differ from, or are similar to, those observed...
Source: NIH Funding Opportunities (Notices, PA, RFA) - Category: Research Source Type: funding
This article provides a review of the preclinical and clinical pharmacology of lorcaserin, including its pharmacokinetic and safety profiles. Preclinical studies with lorcaserin initially focused on simple measures of food intake and body weight gain, but have now expanded to include studies on its effects on appetitive aspects of feeding behaviour and models of binge-eating. A significant number of studies have also shown that lorcaserin alters behaviours related to drug use and addiction, in rodents and non-human primates. Potential clinically-relevant effects of lorcaserin have also been reported in models of pain and s...
Source: Pharmacology and Therapeutics - Category: Drugs & Pharmacology Source Type: research
What is Red Ribbon Week? Red Ribbon Week is October 23-31, a time to get involved in the fight against drugs and bullying. This week-long campaign is celebrated in schools nationwide and provides an opportunity for parents, students, and concerned individuals to take a stand against the violence and drug dependency that can wreak havoc on communities and futures alike. The History of the Red Ribbon Campaign Red Ribbon Week began with the nonprofit organization National Family Partnership (NFP) as a result of the tragic murder of an undercover narcotics officer. In 1985, DEA agent Enrique Camarena was tortured to death by ...
Source: Cliffside Malibu - Category: Addiction Authors: Tags: Addiction Substance Abuse bullying child development children drug abuse and prevention drug addiction drugs red ribbon week Source Type: blogs
The Oregon Court of Appeals on Thursday temporarily halted new rules banning the sale of flavored vaping products, just two days after they went into effect. Gov. Kate Brown had ordered the ban to prevent youth and adults from exposure to health risks from vaping products and nicotine addiction. At least 33 people have died and nearly 1,500 have fallen ill from a vaping-related lung condition. In Oregon, 11 cases have been reported, two of which resul ted in death. The Oregon Health Authority put…
Source: bizjournals.com Health Care:Pharmaceuticals headlines - Category: Pharmaceuticals Authors: Source Type: news
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