Phenotypic and genotypic characteristics of Brucella isolates from the Republic of Kazakhstan

AbstractThe purposes of this study were to determine phenotypic and genotypic characteristics ofBrucella isolates from the Republic of Kazakhstan and to determine their biotype. The focus was laid on culture-morphological, biochemical, and biological properties of 59Brucella isolates from primary cultures. Material was isolated from blood and tissue of serum-positive killed, dead diseased, or aborted domestic cattle from different regions of Kazakhstan where brucellosis is a common problem. Multiple-locus variable number tandem repeat analysis (MLVA) of all strains, isolated in different regions, has shown thatBrucella isolates from the epizootic form two clusters. Based on the comparison with strains available in the MLVA database,B. abortus 0015/B is alike theB. abortus strains isolated from Italy and Portugal.B. melitensis 0016/B isolated from the Almaty region fits the third cluster and is alike theB. melitensis strains isolated from humans in Turkey, China, and Portugal. More than 90% of the overallB. abortus samples were isolated from the northern regions of the East and West Kazakhstan, whileB. melitensis strains were registered in the southeast Kazakhstan. The most frequently recordedB. abortus biovar is biovar 3. The most frequently recordedB. melitensis biovars are biovars 1 and 3.Significance and impact of studyThese results contribute to a better understanding of the geographic pattern ofBrucella infection in Kazakh cattle also important for developing the specifi...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research