Impairment of Mitochondrial-Nuclear Cross Talk in Lymphocytes Exposed to Landfill Leachate.

Impairment of Mitochondrial-Nuclear Cross Talk in Lymphocytes Exposed to Landfill Leachate.
Environ Health Insights. 2019;13:1178630219839013
Authors: Gupta P, Bhargava A, Kumari R, Lodhi L, Tiwari R, Gupta PK, Bunkar N, Samarth R, Mishra PK
Abstract
Landfill leachate, a complex mixture of different solid waste compounds, is widely known to possess toxic properties. However, the fundamental molecular mechanisms engaged with landfill leachate exposure inducing cellular and sub-cellular ramifications are not well explicated. Therefore, we aim to examine the potential of leachate to impair mitochondrial machinery and its associated mechanisms in human peripheral blood lymphocytes. On assessment, the significant increase in the dichlorofluorescein (DCF) fluorescence, accumulation of 8-Oxo-2'-deoxyguanosine (8-oxo-dG), and levels of nuclear factor erythroid 2-related factor 2 (Nrf-2) strongly indicated the ability of the leachate to induce a pro-oxidant state inside the cell. The decrease in the mitochondrial membrane potential and alterations in the mitochondrial genome observed in leachate-exposed cells further suggested the disturbances in mitochondrial machinery. Moreover, these mitochondrial-associated redox imbalances were accompanied by the increased level of NF-κβ, pro-inflammatory cytokines, and DNA damage. In addition, the higher DNA fragmentation, release of nucleosomes, levels of polyadenosine diphosphate ADP-ribose polymera...

Source: Environmental Health Insights - June 8, 2019 Category: Environmental Health Authors: Gupta P, Bhargava A, Kumari R, Lodhi L, Tiwari R, Gupta PK, Bunkar N, Samarth R, Mishra PK Tags: Environ Health Insights Source Type: research