Vitamin D3 increases the Caspase-3 p12, MTHFR, and P-glycoprotein reducing amyloid-β42 in the kidney of a mouse model for Down syndrome

Publication date: Available online 6 June 2019Source: Life SciencesAuthor(s): Fabiana de Campos Gomes, João Simão de Melo-Neto, Merari de Fátima Ramires Ferrari, Carla Patrícia Carlos, Eny Maria Goloni-Bertollo, Érika Cristina PavarinoAbstractAimsRenal dysfunction has been reported in individuals with Down syndrome (DS); however, the causes and mechanisms involved remain unknown. Here, we present a proposal for how the triplication of the amyloid beta precursor protein (APP) and, mainly the amyloid β peptide 1–42 (Aβ42) can favor the development of renal abnormalities in DS. We evaluated the effects of vitamin D3 (VD3) supplementation on morphofunctional aspects and the repercussions on the presence and localization of Aβ42, methylenetetrahydrofolate reductase (MTHFR), caspase-3 p12, and P-glycoprotein (Pgp) in the renal tissue of DS mouse model.Main methodsTwenty female mice (14-week-old) belonging to the B6EiC3Sn-Rb(12.Ts171665Dn)2Cje/CjeDnJ lineage were divided into four experimental groups (n = 5/group): common diet; trisomy (Ts) and wild-type (Wt); and high doses VD3, Ts(VD3), and Wt(VD3). All the groups were treated for 10 weeks. At 24 weeks, the protocol experimental was interrupted. The kidney was weighed, collected, and processed for immunochemical analysis for Aβ42, Caspase-3 p12, MTHFR, and Pgp proteins. All data were analyzed statistically.Key findingsOur results showed that VD3 promoted an increase in caspase-3 p12, MTHFR, and Pgp, and conseque...
Source: Life Sciences - Category: Biology Source Type: research