Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019.

Chlamydia trachomatis samples testing falsely negative in the Aptima Combo 2 test in Finland, 2019.
Euro Surveill. 2019 May;24(22):
Authors: Rantakokko-Jalava K, Hokynar K, Hieta N, Keskitalo A, Jokela P, Muotiala A, Jokiranta TS, Kuusela R, Sarkkinen H, Aittoniemi J, Vuorinen T, Hakanen AJ, Puolakkainen M
Abstract
Since February 2019, over 160 Chlamydia trachomatis (CT) cases testing negative or equivocal by Aptima Combo 2 (AC2) but positive by Aptima CT test run with Panther instruments occurred in Finland. The AC2 test targets chlamydial 23S rRNA while the CT test targets 16S rRNA. Sequencing of 10 strains revealed a nucleotide substitution in 23S rRNA. The significance of this for the failure of the AC2 test to detect the variant is not yet known.
PMID: 31164192 [PubMed - in process]

Source: Euro Surveill - April 30, 2019 Category: Infectious Diseases Authors: Rantakokko-Jalava K, Hokynar K, Hieta N, Keskitalo A, Jokela P, Muotiala A, Jokiranta TS, Kuusela R, Sarkkinen H, Aittoniemi J, Vuorinen T, Hakanen AJ, Puolakkainen M Tags: Euro Surveill Source Type: research