3D printed polyamide macroencapsulation devices combined with alginate hydrogels for insulin-producing cell-based therapies

This study evaluates the biological effect of the macroencapsulation deviceś surface properties (hydrophilicity and porosity). We studied two different pore sizes and hydrophilicities in four different devices containing rat INS1E β-cells embedded in alginate hydrogels. All the devices showed great biocompatibility, although the hydrophilic ones exhibited higher fibroblast adhesion, which could potentially enhance the fibrotic response when implanted. Importantly, INS1E cells did not escape from the devices, denoting high biosafety. Cells grown within all devices and maintained their insulin secretory function. However, the hydrophobic device with a smaller pore size showed better cell viability values and, therefore, it might be the best candidate for the development of a safe β-cell replacement therapy in T1DM.Graphical abstract
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research

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Conclusions Supporting the mechanisms of NO bioavailability via exogenous application of iNOS and L-arginine significantly attenuated I/R-induced alterations in a skin flap rat model. This pharmacologic preconditioning could be an easy and effective interventional strategy to uphold conversation of L-arginine to NO even on ischemic and type 1 diabetic conditions.
Source: Annals of Plastic Surgery - Category: Cosmetic Surgery Tags: Research Source Type: research
Fight Aging! publishes news and commentary relevant to the goal of ending all age-related disease, to be achieved by bringing the mechanisms of aging under the control of modern medicine. This weekly newsletter is sent to thousands of interested subscribers. To subscribe or unsubscribe from the newsletter, please visit: https://www.fightaging.org/newsletter/ Longevity Industry Consulting Services Reason, the founder of Fight Aging! and Repair Biotechnologies, offers strategic consulting services to investors, entrepreneurs, and others interested in the longevity industry and its complexities. To find out m...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
CONCLUSIONS T1DM recurrence after pancreas transplantation is infrequent; however, it is one of the causes of pancreatic graft loss that should always be ruled out. Negative autoimmunity prior to transplantation does not ensure that T1DM does not recur. PMID: 31767825 [PubMed - in process]
Source: Annals of Transplantation - Category: Transplant Surgery Authors: Tags: Ann Transplant Source Type: research
Publication date: Available online 19 November 2019Source: Chemical Engineering Research and DesignAuthor(s): Katarzyna Skrzypek, Efrem Curcio, Dimitrios StamatialisAbstractWidespread application of islet transplantation as a cure for type 1 Diabetes Mellitus patients is limited mainly due to the need for life-long immunosuppression associated with serious side effects. Immunoisolation by encapsulation of islets in semi-permeable membranes may overcome this issue as it allows for transplantation potentially obviating the need for immunosuppressive drug therapy.This work focuses on the development and validation of a model ...
Source: Chemical Engineering Research and Design - Category: Chemistry Source Type: research
Conclusion: In a representative Brazilian population of T1DM patients, those with IDAA1c ≤ 9 presented a lower frequency of MC, as well as fewer episodes of hypoglycemia, in the month prior to the analysis.
Source: Frontiers in Endocrinology - Category: Endocrinology Source Type: research
This study aims at the fabrication and evaluation of alginate-chitosan microcapsules encapsulating insulin-secreting beta-TC-6 cells using an automated spraying nozzle. Uniform spherical microcapsules (250-350 µm) encapsulated with beta-TC-6 cells were fabricated in large quantities in a short span of time. Microencapsulated beta-TC-6 cells were transplanted intraperitoneally into streptozotocin (STZ) induced diabetic mice and monitored for immune tolerance and decrease in blood glucose levels. Mice that received microencapsulated beta-TC-6 cells maintained normoglycemia for 35±5 days before rejection. However...
Source: International Journal of Pharmaceutics - Category: Drugs & Pharmacology Source Type: research
Kidney transplantation is the treatment of choice for most patients with end-stage renal disease, and pancreas transplantation is the only treatment for type 1 diabetes mellitus that can induce insulin-independent normoglycemia.
Source: Medscape Today Headlines - Category: Consumer Health News Tags: Resource Center Source Type: news
AbstractPancreas transplantation is considered the curative treatment for severe type 1 diabetes mellitus in selected cases. Since the first procedure in 1966, surgical techniques have been improved. The current trend among most medical centers, as well as at our Institution, is enteric drainage and systemic venous or portal anastomosis. The aim of this pictorial essay is to describe the main imaging features of pancreatic transplantation with duodenoduodenostomy drainage.
Source: Abdominal Imaging - Category: Radiology Source Type: research
AbstractAimsTo investigate the long ‐term efficacy and safety of Islet transplantation (ITx) compared to multiple daily injections (MDI) or continuous subcutaneous insulin infusion (CSII).MethodsAmong 619 patients diagnosed as insulin ‐dependent diabetes mellitus (IDDM) or type 1 diabetes in our hospital, seven patients were selected as the ITx group and 26 age‐matched patients with no endogenous insulin secretion were selected as the MDI/CSII group. HbA1c, AST/ALT and creatinine were assessed retrospectively at 1, 2, 5 and 1 0 years for both groups; serum C‐peptide immunoreactivity (CPR) was assessed for the ITx g...
Source: Journal of Diabetes Investigation - Category: Endocrinology Authors: Tags: Original Article Source Type: research
Boron containing compounds promote the survival and the maintenance of pancreatic β-cells. Mol Biol Rep. 2019 Jul 31;: Authors: Aydın S, Demirci S, Doğan A, Sağraç D, Kaşıkcı E, Şahin F Abstract Diabetes mellitus is worldwide disease. The life of diabetic patients are dependent on exogenous insulin. Pancreas or particularly islet transplantations are performed for reducing external insulin dependency. External substances are also used to protect the β-cells from the death or increase insulin secretion. In the current study, two different boron containing compounds (sodium penta...
Source: Molecular Biology Reports - Category: Molecular Biology Authors: Tags: Mol Biol Rep Source Type: research
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