Key sites insight on the stereoselectivity of four mined aldo-keto reductases toward α-keto esters and halogen-substituted acetophenones.

In this study, four novel aldo-keto reductases with significant activity and stereoselectivity toward a variety of α-keto esters and halogen-substituted acetophenones were identified by genome mining. Through analysis of the crystal structure and multiple-sequence alignment of the starting AKR YvgN from Bacillus subtilis, residues F25 and W113 were proposed as the key positions that might control the stereoselectivity of YvgN. F25S and F25S/W113F variants of YvgN were able to improve its activity and stereoselectivity toward some α-keto ester compounds and halogen-substituted acetophenone derivatives. In addition, similar enhancement of catalytic activity and stereoselectivity was also found in the other three AKRs with corresponding mutations of starting YvgN.
PMID: 31165224 [PubMed - as supplied by publisher]

Source: Applied Microbiology and Biotechnology - June 3, 2019 Category: Microbiology Authors: Zhang W, Zhu T, Li H, Qin F, Zhang F, Zhang R, Jia X, Qin B, You S Tags: Appl Microbiol Biotechnol Source Type: research