Renal nerves and leukocyte infiltration in the kidney during salt-sensitive hypertension.

Renal nerves and leukocyte infiltration in the kidney during salt-sensitive hypertension. Am J Physiol Regul Integr Comp Physiol. 2019 Jun 05;: Authors: Alsheikh AJ, Lund H, Dasinger JH, Abais-Battad JM, Fehrenbach DJ, Mattson DL Abstract Based on previous studies suggesting a role of renal nerves in renal inflammation, the present studies were performed to test the hypothesis that renal nerves mediate renal damage in Dahl salt-sensitive (SS) hypertension by increasing renal leukocyte infiltration. Experiments were performed in Dahl SS rats using both bilateral renal denervation (RDN) or bilateral sham operation (n=10-11 per group); and using unilateral RDN and contralateral sham (n=10). Following denervation, rats were switched from a low salt 0.4% NaCl (LS) to a high salt 4% NaCl (HS) diet and maintained on HS for twenty-one days. Bilateral RDN reduced the magnitude of hypertension assessed by radio-telemetry in Dahl SS rats compared to sham (mean arterial pressure 140.9±4.8 mmHg vs 159.7±3.5 mmHg respectively), and reduced proteinuria at day 21 of HS. However, assessment of renal leukocyte infiltration demonstrated no significant effect of bilateral RDN on the number of infiltrating total leukocyte (3.6±0.5 in RDN vs 4.3±0.3 x106 CD45+ cells in sham) or any of the subsets examined by flow cytometry. The unilateral RDN experiment showed no effect of RDN on the renal infiltration of leukocytes (6.5.6±0.9 x106 in RDN vs 6.1±1.1...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - Category: Physiology Authors: Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research