Proof of concept for a portable platform for molecular diagnosis of tropical diseases: On-chip ready-to-use qPCR for detection of Trypanosoma cruzi or Plasmodium spp

Although molecular diagnostics is well established in clinical laboratories, its full potential has not been extended to field settings. Typically, diagnostic qPCR reagents require temperature-controlled transportation and storage. Furthermore, thermocyclers are bulky and fragile, requiring good infrastructure for optimal operation. These major hurdles strongly limit the use of molecular-based tests in low-resource scenarios. Here, qPCR was used for detection of Trypanosoma cruzi or Plasmodium spp.

Source: Journal of Molecular Diagnostics - June 3, 2019 Category: Pathology Authors: Rita C.P. Rampazzo, Ana C. Graziani, Keren K. Leite, Jhully A. Surdi, Cheysa A. Biondo, Maykon L.N. Costa, Thiago Jacomasso, Marco Cereda, Marco De Fazio, Marco A. Bianchessi, Otac ílio C. Moreira, Constança Britto, Joana D.N. Costa, Viviane M. Góes, A Tags: Regular Article Source Type: research