The design and application of a bacterial ghost vaccine to evaluate immune response and defense against avian pathogenic Escherichia coli O2:K1 serotype

Publication date: Available online 4 June 2019Source: Research in Veterinary ScienceAuthor(s): Reza Hoseini Shahidi, Gholamreza Hashemi Tabar, Mohammad Reza Bassami, Abdollah Jamshidi, Hesam DehghaniAbstractAn Escherichia coli (E. coli) O2:K1 bacterial ghost was produced by controlled expression of bacteriophage PhiX 174 lysis gene E. Temperature controlled expression of this gene caused tunnels and holes in the cell wall of E. coli O2:K1 bacterium, leading to loss of cytoplasmic contents. Formation of E. coli O2:K1 ghost was confirmed by scanning electron microscopy and determination of colony forming units. To evaluate the efficiency of this bacterial ghost vaccine to elicit cellular and humoral immune responses, 85 one day old chickens from Ross 308 breed were divided into the following 5 groups; group 1 (non-immunized control), group 2 (vaccine administered by injection of E. coli O2:K1 killed vaccine), group 3 (vaccine administered by injection of E. coli O2:K1 ghost), group 4 (vaccine administered by inhalation of E. coli O2:K1 ghost), and group 5 (neither immunized, nor challenged as negative control). The groups of 2, 3, and 4 were received vaccines at days 7, 14, and 22. Groups 1 to 4 were challenged with the wild type at day 33. Evaluation of post-mortem lesions and immune responses in all groups showed that chicken injected with the killed vaccine and the bacterial ghost had the best protection. These findings suggest that this bacterial ghost has the potential to ...
Source: Research in Veterinary Science - Category: Veterinary Research Source Type: research

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In conclusion, our results show for the first time that CATH-B1 has several immunomodulatory activities and thereby could be an important factor in the chicken immune response.
Source: Veterinary Research - Category: Veterinary Research Source Type: research
to J Abstract Pathogenic strains of Escherichia coli may invade the subcutaneous tissue of poultry and cause cellulitis, whilst the pathogen may also cause lesions in internal organs such as the liver. Current paper co-relates Escherichia coli and virulence genes characteristic of Avian Pathogenic Escherichia coli (APEC) in broilers´ cellulitis and liver lesions. One hundred carcasses were retrieved from the production chain in an avian abattoir in the state of Bahia, Brazil, between August 2013 and January 2014, due to detection of cellulitis lesions. Cellulitis and liver samples were retrieved aseptically ...
Source: Braz J Biol - Category: Biology Authors: Tags: Braz J Biol Source Type: research
Microbial Drug Resistance, Ahead of Print.
Source: Microbial Drug Resistance - Category: Microbiology Authors: Source Type: research
In conclusion, the overall agreement between the four different testing methodologies was very high. These results confirm the reliability of the disk diffusion and gradient strip test methods as substantiated alternatives, next to the gold standard agar and microbroth dilution, for fluoroquinolone susceptibility testing of APEC isolates.
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
AbstractEscherichia coli (E.coli) is a normal intestinal microflora of birds, including ostrich. However, some strains are pathogenic to ostrich. There is a lack of knowledge concerning the differences between commensal and pathogenicE.coli isolates of ostrich. This research aimed to characterize the antimicrobial susceptibility profile and virulence gene content of presumptive commensal and pathogenicE.coli isolates of ostrich. A total of 129 bacterial isolates (commensal strains no. = 45; pathogenic strains no. = 84) were obtained from ostriches. The resistance profile of these isolates was investigated by the disk diffu...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research
This study argues that Congress should repeal all federal aid-to-state programs for many reasons, including that aid comes with costly strings attached that destroy local democracy.Richard Epstein and Mario Loyolanoted about aid programs: “When Americans vote in state and local elections, they think they are voting on state and local policies. But often they are just deciding which local officials get to implement the dictates of distant and insulated federal bureaucrats, whom even Congress can’t control.”I came across a table (p. 82) in New Jersey ’s budget that lists the $15 billion the state rece...
Source: Cato-at-liberty - Category: American Health Authors: Source Type: blogs
This study revealed the feasibility of a novel functional antibody fragment development strategy by generating diversified avian IgY-scFv libraries towards the pathogenic target of interest for both detection and therapeutic purposes in veterinary medicine.
Source: Veterinary Research - Category: Veterinary Research Source Type: research
Abstract The aim of this study was to explore the between-group and temporal variations in the intestinal Escherichia coli populations of broilers under experimental conditions, taking both antimicrobial resistance and virulence into consideration. Four replicates of 45 commercial chicks were reared in four animal facilities. On their first day of life (Day 0), they were orally inoculated with two extended-spectrum-cephalosporin-resistant (ESCR) E. coli (2.72 log10 CFU of a bla CMY-2- and 2.55 log10 CFU of a bla CTX-M-carrying E. coli). Faecal samples were then collected weekly and caecal samples were obtained fro...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
Abstract Spotty Liver Disease (SLD) is an emerging disease of serious concern in the egg production industry, as it causes significant egg loss and mortality in layer hens. The causative agent is a newly identified Gram-negative bacterium, Campylobacter hepaticus, and knowledge about C. hepaticus pathogenesis and the potential for vaccine development is still in its infancy. Current detection methods for SLD, such as PCR and culturing, only detect an active infection and will not give any indication of a past infection from which the bacteria have been cleared. An immunological assay, on the other hand, can provid...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research
Abstract Avian pathogenic Escherichia coli (APEC) are major bacterial pathogens of commercial poultry contributing to extensive economic losses and contamination of the food chain. One of the initial steps in bacterial infection and successful colonization of the host is adhesion to the host cells. A random transposon mutant library (n=1300) of APEC IMT 5155 was screened phenotypically for adhesion to chicken (CHIC-8E11) and human (LoVo) intestinal epithelial cell lines. The detection and quantification of adherent bacteria was performed by a modified APEC-specific antibody staining assay using fluorescence micros...
Source: Applied and Environmental Microbiology - Category: Microbiology Authors: Tags: Appl Environ Microbiol Source Type: research
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