MTB driven B cells producing IL-35 and secreting high level of IL-10 in the patients with active pulmonary tuberculosis

Publication date: August 2019Source: Molecular Immunology, Volume 112Author(s): You-Chao Dai, Wan-Dang Wang, Jun-Ai Zhang, Chen Chen, Hou-Long Luo, Huan Xu, Ying Peng, Hong Luo, Xu-Ran Yang, Xinchun Chen, Xian-Jin Wu, Guang-Hui Chen, Zheng W. Chen, Jun-Fa XuAbstractRegulatory B cells (Bregs) have critical roles as a negative regulator of immunity, mainly due to the fact that it secrets high a level of interleukin 10 (IL-10). Recently, a new subset of Bregs was identified as a key source of IL-35, which is an immunosuppressive cytokine and conventionally thought to be secreted by regulatory T cells (Tregs). Our previous study showed that the level of IL-35 in serum was elevated in the patients with active tuberculosis (ATB). However, none of the studies reported that IL-35 is secreted by B cells in ATB patients. In the current study, we found that the mRNA expressions of the both subunits (p35 and Ebi3) of IL-35 by circulating B cells were increased in ATB patients. By using immunohistochemistry and immunofluorescence staining, we found a subset of B cells infiltrated into the tuberculous granuloma of ATB patients also expressed IL-35. Moreover, Mycobacterium tuberculosis (MTB) lysate stimulation assay also demonstrated higher levels of IL-35 were exerted by MTB lysate within purified B cells from healthy control group (HC). Flow cytometry analysis further showed that the IL-35-producing B cells from ATB patients produced a higher level of IL-10. Taken together, IL-35-producin...
Source: Molecular Immunology - Category: Allergy & Immunology Source Type: research