Near-infrared Fluorescent Peptides with High Tumor Selectivity: Novel Probes for Image-Guided Surgical Resection of Orthotopic Glioma

Conclusions: NIRF imaging using Cy5.5-RKL is promising not only for diagnostic purposes but also for use in image-guided surgery for orthotopic glioblastoma or other superficial tumors. Key Words: Near-infrared fluorescence imaging, glioblastoma, RGD, NGR, anti-cancer peptide Suppl. Fig. 1: Confocal microscopy images of U87MG cells incubated with Cy5.5-RKL (50 nM of Cy5.5 labeled peptides in 500 μL of PBS) (magnification x60). For the blocking group, the U87MG cells were co-incubated with extra unlabeled peptide (50 μM). The results were scanned under confocal laser-scanning microscopy. Suppl. Fig. 2: In vivo fluorescent imaging of the probes. The mice received 1.5 nmol of Cy5.5-RKL intravenously and subjected to optical imaging at various time points post injection. Suppl. Fig. 3: Time-course fluorescence imaging of the orthotopic glioma mouse model. a: fluorescence images of Cy5.5-RKL in the orthotopic U87MG glioblastoma model from 0.5 to 8h post injection. b: in vivo targeting quantification of Cy5.5-RKL in orthotopic U87MG glioblastoma vs. muscle. c: tumor contrast (tumor-to-muscle) calculated from ROI measurement at different time points after administration of Cy5.5-RKL. Suppl. Fig. 4: Near-infrared fluorescence imaging for the delineation of boundary of glioma. a: NIRF imaging delineates boundary of glioblastoma and normal brain tissue at 8 h after intravenous injection of Cy5.5-RKL. The tumor can be clearly visualized in situ through NIRF imaging. b: H&E stainin...
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Novel Multimodality and Nonradioactive Probes Posters Source Type: research

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Daria Riva Neurofibromatosis type-1 (NF1) is a monogenic tumor-predisposition syndrome creating a wide variety of cognitive and behavioral abnormalities, such as decrease in cognitive functioning, deficits in visuospatial processing, attention, and social functioning. NF1 patients are at risk to develop neurofibromas and other tumors, such as optic pathway gliomas and other tumors of the central nervous system. Few studies have investigated the impact of an additional diagnosis of brain tumor on the cognitive outcome of children with NF1, showing unclear results and without controlling by the effect of surgery, radio-...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research
Authors: Tsiouris S, Bougias C, Fotopoulos A Abstract Cerebral gliomas comprise a heterogeneous group of primary neoplasms of the central nervous system, representing a significant cause of cancer morbidity and mortality. Contrast-enhanced magnetic resonance imaging (MRI) is paramount for identifying structural brain abnormalities related to the development of gliomas. Although morphological MRI remains the current standard of care for initial diagnostic workup, surgical planning, monitoring therapy response and surveillance during follow-up, it is rather difficult to define tumor grade and boundaries and to assess...
Source: Hellenic Journal of Nuclear Medicine - Category: Nuclear Medicine Tags: Hell J Nucl Med Source Type: research
Abstract13N-Ammonia (13N-NH3) is widely used positron emission tomography/computed tomography (PET/CT) radiotracer for the measurement of myocardial blood perfusion; the possible role of13N-NH3 PET or PET/CT in oncological disease is not yet clear. Aim of this review is to evaluate the diagnostic performances of13N-NH3 PET in this field. A comprehensive computer literature search of the PubMed/MEDLINE, Scopus, and Embase databases was conducted including articles up to June 2019. Eighteen articles were finally included in the review. From the analyses of the selected studies, the following main findings could be drawn: (1)...
Source: Japanese Journal of Radiology - Category: Radiology Source Type: research
ConclusionsThese findings provide evidence from molecular, cellular, and physiological levels that strongly suggest that CC12 is a promising small-molecule agent for human GBM.Legal entity responsible for the studyThe author.FundingHas not received any funding.DisclosureThe author has declared no conflicts of interest.
Source: Annals of Oncology - Category: Cancer & Oncology Source Type: research
Conclusion The LoG-Glio registry has been successfully implemented. Applied selection criteria result in an appropriately balanced patient cohort. Short-term outcome data on epidemiology as well as the influence of current surgical techniques and adjuvant treatment on patient outcomes are expected. In the long run, the aim of the registry is to validate the new molecular-based WHO classification and the influence of the extent of resection on progression-free survival and OS. The registry provides an open platform for future research projects benefiting patients with LGG. Trial registration NCT02686229 Clinica...
Source: Journal of Neurological Surgery Part A: Central European Neurosurgery - Category: Neurosurgery Authors: Tags: Original Article Source Type: research
CONCLUSION: Our results suggest that our algorithm can non-invasively predict the 1p/19q status of presumed LGG with a performance that on average outperformed the oncological neurosurgeons . Evaluation on an independent dataset indicates that our algorithm is robust and generalizable. PMID: 31548344 [PubMed - as supplied by publisher]
Source: Clinical Cancer Research - Category: Cancer & Oncology Authors: Tags: Clin Cancer Res Source Type: research
Conclusions: The present study demonstrates that hyperpolarized 13C MRS potentially can be used to identify and characterize gliomas that are 18F-FDG negative. The Lac/Bic imaging marker is interesting because it reports on the ratio between increased glycolysis (Warburg effect) and the more ATP favorable oxidative phosphorylation inside the cells3. Further histological analysis will be performed to get better understanding how the metabolic differences correlate with the proliferative state of the tumor. Acknowledgements NIH grants R01CA176836, R01EB019018, P41EB01589, S10OD012283, S10OD018130-01 GE HealthcareLucas Founda...
Source: Journal of Nuclear Medicine - Category: Nuclear Medicine Authors: Tags: Novel Multimodality and Nonradioactive Probes Posters Source Type: research
Yong Zhao1, Peijuan Liu1, Zhiqian Xin1, Changhong Shi1, Yinlan Bai2, Xiuxuan Sun3, Ya Zhao1, Xiaoya Wang1,4, Li Liu1,5, Xuan Zhao1,4, Zhinan Chen3* and Hai Zhang1,6* 1Laboratory Animal Center, Air Force Medical University, Xi’an, China 2Department of Microbiology, Air Force Medical University, Xi’an, China 3Department of Cell Biology, National Translational Science Center for Molecular Medicine, Air Force Medical University, Xi’an, China 4College of Veterinary Medicine, Northwest A&F University, Yangling, China 5Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Nor...
Source: Frontiers in Genetics - Category: Genetics & Stem Cells Source Type: research
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Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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Source: Frontiers in Oncology - Category: Cancer & Oncology Source Type: research
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