Near-infrared Fluorescent Peptides with High Tumor Selectivity: Novel Probes for Image-Guided Surgical Resection of Orthotopic Glioma

Conclusions: NIRF imaging using Cy5.5-RKL is promising not only for diagnostic purposes but also for use in image-guided surgery for orthotopic glioblastoma or other superficial tumors. Key Words: Near-infrared fluorescence imaging, glioblastoma, RGD, NGR, anti-cancer peptide Suppl. Fig. 1: Confocal microscopy images of U87MG cells incubated with Cy5.5-RKL (50 nM of Cy5.5 labeled peptides in 500 μL of PBS) (magnification x60). For the blocking group, the U87MG cells were co-incubated with extra unlabeled peptide (50 μM). The results were scanned under confocal laser-scanning microscopy. Suppl. Fig. 2: In vivo fluorescent imaging of the probes. The mice received 1.5 nmol of Cy5.5-RKL intravenously and subjected to optical imaging at various time points post injection. Suppl. Fig. 3: Time-course fluorescence imaging of the orthotopic glioma mouse model. a: fluorescence images of Cy5.5-RKL in the orthotopic U87MG glioblastoma model from 0.5 to 8h post injection. b: in vivo targeting quantification of Cy5.5-RKL in orthotopic U87MG glioblastoma vs. muscle. c: tumor contrast (tumor-to-muscle) calculated from ROI measurement at different time points after administration of Cy5.5-RKL. Suppl. Fig. 4: Near-infrared fluorescence imaging for the delineation of boundary of glioma. a: NIRF imaging delineates boundary of glioblastoma and normal brain tissue at 8 h after intravenous injection of Cy5.5-RKL. The tumor can be clearly visualized in situ through NIRF imaging. b: H&E sta...

Source: Journal of Nuclear Medicine - May 20, 2019 Category: Nuclear Medicine Authors: Yang, W., Ma, W., Liu, Y., Wang, J. Tags: Novel Multimodality and Nonradioactive Probes Posters Source Type: research