Profilin2 is required for filamentous actin formation induced by human parainfluenza virus type 2.

Profilin2 is required for filamentous actin formation induced by human parainfluenza virus type 2.
Virology. 2019 May 24;533:108-114
Authors: Ohta K, Matsumoto Y, Nishio M
Abstract
We previously reported that human parainfluenza virus type 2 (hPIV-2) promoted RhoA activation and subsequent filamentous actin (F-actin) formation. Actin-binding proteins, such as profilin and cofilin, are involved in the regulation of F-actin formation by RhoA signaling. In the present study, we identified profilin2 as a key molecule that is involved in hPIV-2-induced F-actin formation. Immunoprecipitation assays demonstrated that hPIV-2 V protein binds to profilin2 but not to profilin1. Mutation of Trp residues within C-terminal region of V protein abolished the binding capacity to profilin2. Depletion of profilin2 resulted in the inhibition of hPIV-2-induced F-actin formation and the suppression of hPIV-2 growth. Overexpression of wild type V but not Trp-mutated V protein reduced the quantity of actin co-immunoprecipitated with profilin2. Taken together, these results suggest that hPIV-2 V protein promotes F-actin formation by affecting actin-profilin2 interaction through its binding to profilin2.
PMID: 31150988 [PubMed - as supplied by publisher]

Source: Virology - May 23, 2019 Category: Virology Authors: Ohta K, Matsumoto Y, Nishio M Tags: Virology Source Type: research