Tuning probe selectivity for chemical proteomics applications.

Tuning probe selectivity for chemical proteomics applications.
Curr Opin Chem Biol. 2012 Dec 27;
Authors: Haedke U, Küttler EV, Vosyka O, Yang Y, Verhelst SH
Abstract
Covalent chemical probes enable investigation of a desired fraction of the proteome. It is possible to adjust the selectivity of these probes, so they either react with a certain amino acid in all proteins, a class of proteins or only a single protein species. A combination of specific reactive groups with additional recognition elements can fine tune probes to hit the desired proteins, even in the presence of related family members. Using probes of lower or higher selectivity, screening experiments for inhibitor discovery and imaging experiments for localization studies can be performed, showing only a fraction of the power of covalent small molecule probes.
PMID: 23273613 [PubMed - as supplied by publisher]

Source: Current Opinion in Chemical Biology - December 27, 2012 Category: Biochemistry Authors: Haedke U, Küttler EV, Vosyka O, Yang Y, Verhelst SH Tags: Curr Opin Chem Biol Source Type: research